Uzbay I T, Usanmaz S E, Tapanyigit E E, Aynacioglu S, Akarsu E S
Department of Pharmacology, Faculty of Medicine, Gülhane Military Medical Academy, Ankara, Turkey.
Drug Alcohol Depend. 1998 Dec 1;53(1):39-47. doi: 10.1016/s0376-8716(98)00102-1.
Changes in dopaminergic and serotonergic levels and metabolites in cerebral cortex, corpus striatum and hippocampus were investigated during the first 6-h of withdrawal in ethanol-dependent Wistar rats. Ethanol was given by a liquid diet for 21 days. The concentration of ethanol was 7.2% (v/v) for the last 15 days of the exposure. After 2, 4 and 6 h of ethanol withdrawal, and after audiogenic stimulus (100 dB for 60 s) at 6 h of ethanol withdrawal, various brain regions were assayed for levels of dopamine (DA), DOPAC, HVA, serotonin (5-HT) and 5-HIAA. Behavioral signs of ethanol withdrawal and blood ethanol levels were also evaluated in other parallel groups of ethanol-dependent rats. Significant decreases in 5-HT levels and significant increases in HVA levels in striatum were found during the first 6 h of ethanol withdrawal and after the audiogenic seizures. In hippocampus, 5-HIAA levels were significantly reduced after 2 h of ethanol withdrawal and after the audiogenic seizures. 5-HIAA levels significantly increased after 2 h of ethanol withdrawal in cerebral cortex. Significant increases in both DA and 5-HT levels were also found in cerebral cortex after the audiogenic seizures. The results suggest that the levels of DA, 5-HT and their metabolites are altered by ethanol withdrawal. Furthermore, this may suggest that DA and 5-HT may be involved in the first 6 h of ethanol withdrawal syndrome in rats.
在乙醇依赖的Wistar大鼠戒断的最初6小时内，研究了大脑皮层、纹状体和海马体中多巴胺能和5-羟色胺能水平及代谢产物的变化。通过液体饮食给予乙醇，持续21天。在暴露的最后15天，乙醇浓度为7.2%（体积/体积）。在乙醇戒断2、4和6小时后，以及在乙醇戒断6小时给予听源性刺激（100分贝，持续60秒）后，检测各个脑区的多巴胺（DA）、3,4-二羟基苯乙酸（DOPAC）、高香草酸（HVA）、5-羟色胺（5-HT）和5-羟吲哚乙酸（5-HIAA）水平。还在其他平行的乙醇依赖大鼠组中评估了乙醇戒断的行为体征和血液乙醇水平。在乙醇戒断的最初6小时内以及听源性惊厥后，纹状体中的5-HT水平显著降低，HVA水平显著升高。在海马体中，乙醇戒断2小时后以及听源性惊厥后，5-HIAA水平显著降低。在大脑皮层，乙醇戒断2小时后，5-HIAA水平显著升高。听源性惊厥后，大脑皮层中的DA和5-HT水平也显著升高。结果表明，乙醇戒断会改变DA、5-HT及其代谢产物的水平。此外，这可能表明DA和5-HT可能参与大鼠乙醇戒断综合征的最初6小时。
