Diaz-Jauanen E, Strickland R G, Williams R C
Am J Med. 1975 May;58(5):620-8. doi: 10.1016/0002-9343(75)90497-0.
Proportions and absolute numbers of T and B lymphocytes were determined among 30 newborn infants and group of 77 elderly patients 60 to 95 years of age. Total lymphocytes in the cord blood of the newborn showed a distinct elevation in total numbers of T and B lymphocytes (p less than 0.005) as compared to that in blood from normal adult controls, reflecting the relative lymphocytosis of infancy. Proportions of cord blood T lymphocytes as reflected by the sheep cell rosette technic were considerable lower than those in lymphocytes from normal adult controls, however, proportions of cord blood T lymphocytes as determined by indirect immunofluorescence were not significantly different from those in controls. Old people showed a significant reduction in total numbers of lymphocytes (p less than 0.005) when compared with those in normal adult controls 18 to 51 years of age. Moreover, there was a significant increase in the relative proportions of peripheral blood B lymphocytes in the elderly although the absolute numbers of B lymphocytes in the elderly although the absolute numbers of B cells did not differ from those in younger controls. A significant decrease in total numbers of T cells as measured both by sheep cell rosettes and indirect immunofluorescence was recorded among older patients (p less than 0.001). In addition, there was a broad increment in the incidence of various autoantibodies (anti-nuclear, andi-IgG, antismooth muscle, antimitochondrial and antiparietal cell) among the old people studies. No direct correlation could be determined between relative B-cell percentage increase or T-cell decrease and the presence of various autoantibodies in individual patients. Diminution in total lymphocyte counts as well as absolute numbers of T cells in the elderly may provide the cellular basis for an increased susceptibility to neoplasia and infection.
在30名新生儿和77名60至95岁老年患者中测定了T淋巴细胞和B淋巴细胞的比例及绝对数量。与正常成人对照组的血液相比，新生儿脐带血中的总淋巴细胞显示T淋巴细胞和B淋巴细胞总数明显升高（p<0.005），这反映了婴儿期的相对淋巴细胞增多。通过绵羊细胞玫瑰花结技术反映的脐带血T淋巴细胞比例显著低于正常成人对照组淋巴细胞中的比例，然而，通过间接免疫荧光测定的脐带血T淋巴细胞比例与对照组无显著差异。与18至51岁的正常成人对照组相比，老年人的淋巴细胞总数显著减少（p<0.005）。此外，老年人外周血B淋巴细胞的相对比例显著增加，尽管老年人B淋巴细胞的绝对数量与年轻对照组无差异。在老年患者中，通过绵羊细胞玫瑰花结和间接免疫荧光测量的T细胞总数均显著减少（p<0.001）。此外，在研究的老年人中，各种自身抗体（抗核抗体、抗IgG抗体、抗平滑肌抗体、抗线粒体抗体和抗壁细胞抗体）的发生率有广泛增加。在个体患者中，无法确定相对B细胞百分比增加或T细胞减少与各种自身抗体的存在之间的直接相关性。老年人总淋巴细胞计数以及T细胞绝对数量的减少可能为肿瘤形成和感染易感性增加提供细胞基础。
