Rheins M S, Filppi J A, Moore V S
Cancer Res. 1975 Jun;35(6):1514-9.
Studies were conducted to determine whether the sulfhydryl inhibitor, sodium iodoacetate, administered to preleukemic AKR mice and to mature C3H mice altered the immunocompetence of their bone marrow. Parameters investigated included the splenic plaque-forming capacity directed to sheep erythrocytes of bone marrow transferred from iodoacetate-treated aanimals to irradiated syngeneic recipients and the mitogenic responsiveness of bone marrow cells from untreated and iodoacetate-treated preleukemic AKR mice to phytohemagglutinin and concanavalin A. The administration of two 0.5-ml doses of 10 mM iodoacetate to preleukemic AKR mice and to C3H mice resulted in a significant increase in bone marrow immunocompetence. Irradiated mice given marrow transplants from iodoacetate-treated syngeneic donors exhibited greater numbers of plaque-forming cells directed against sheep erythrocytes than did recipients of marrow from control animals. This effect was abrogated when the donor marrow was previously treated in vitro with rabbit antimouse brain serum and the complement to remove thymus-derived lymphocytes. The mitogenic responsiveness of marrow cultures from iodoacetate-treated AKR mice to phytohemagglutinin was similar to that observed for control mice, while the response to concanavalin A was decreased. These findings suggest that the administration of iodoacetate potentiated the immunocompetence of bone marrow by affecting thymus-derived cells.
开展了多项研究，以确定向白血病前期AKR小鼠和成熟C3H小鼠施用巯基抑制剂碘乙酸钠是否会改变其骨髓的免疫能力。所研究的参数包括从经碘乙酸钠处理的动物转移至经辐照的同基因受体的骨髓针对绵羊红细胞的脾集落形成能力，以及未处理和经碘乙酸钠处理的白血病前期AKR小鼠的骨髓细胞对植物血凝素和刀豆球蛋白A的促有丝分裂反应性。向白血病前期AKR小鼠和C3H小鼠施用两剂0.5毫升10 mM的碘乙酸钠，导致骨髓免疫能力显著增强。接受经碘乙酸钠处理的同基因供体骨髓移植的辐照小鼠，与接受对照动物骨髓的受体相比，表现出更多针对绵羊红细胞的集落形成细胞。当供体骨髓事先在体外经兔抗小鼠脑血清和补体处理以去除胸腺来源的淋巴细胞时，这种效应被消除。经碘乙酸钠处理的AKR小鼠骨髓培养物对植物血凝素的促有丝分裂反应性与对照小鼠相似，而对刀豆球蛋白A的反应性降低。这些发现表明，碘乙酸钠的施用通过影响胸腺来源的细胞增强了骨髓的免疫能力。
