Playfair J H, Purves E C
Immunology. 1971 Jul;21(1):113-21.
Bone marrow-thymus cooperation experiments were carried out in lethally irradiated mice with sheep red blood cells (SRBC) as the antigen and direct plaque-forming cells (PFC) as the end point. Various parameters were altered, with the following results: (1) Above 800 rad, the response by marrow cells alone, as well as the increase due to added thymus cells, was independent of irradiation dose. (2) The response of marrow cells was greatest at high SRBC concentrations, but the co-operative effect of thymus cells was most evident at lower SRBC levels, and completely absent at high levels. (3) Increasing the number of marrow cells, without thymus, gave increasing numbers of PFC, but the dose-response curve did not suggest cell synergism. (4) Thymectomy and antithymocyte serum treatment of host or donor did not prevent the response by marrow cells alone. It was concluded that this was a true IgM response by antibody-forming precursors from the marrow, unaided by thymus-derived cells.
以绵羊红细胞（SRBC）作为抗原，直接溶血空斑形成细胞（PFC）作为检测终点，在接受致死剂量照射的小鼠身上进行了骨髓-胸腺协作实验。改变了各种参数，得到以下结果：（1）超过800拉德时，单独骨髓细胞的反应以及因添加胸腺细胞而增加的反应与照射剂量无关。（2）骨髓细胞在高SRBC浓度时反应最大，但胸腺细胞的协同效应在较低SRBC水平时最明显，在高浓度时则完全不存在。（3）不添加胸腺细胞而增加骨髓细胞数量，会使PFC数量增加，但剂量反应曲线未显示细胞协同作用。（4）对宿主或供体进行胸腺切除和抗胸腺细胞血清处理并不能阻止单独骨髓细胞的反应。得出的结论是，这是骨髓中抗体形成前体细胞的真正IgM反应，不受胸腺来源细胞的辅助。
