Ip S P, Yiu H Y, Ko K M
Department of Biochemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, P.R. China.
Mol Cell Biochem. 2000 May;208(1-2):151-5. doi: 10.1023/a:1007029625406.
Pretreating mice with schisandrin B (Sch B), a dibenzocyclooctadiene derivative isolated from the fruit of Schisandra chinensis, at a daily dose of 1 mmol/kg for 3 days protected against menadione-induced hepatic oxidative damage in mice, as evidenced by decreases in plasma alanine aminotransferase activity (78%) and hepatic malondialdehyde level (70%), when compared with the menadione intoxicated control. In order to define the biochemical mechanism involved in the hepatoprotection afforded by Sch B pretreatment, we examined the activity of DT-diaphorase (DTD) in hepatocytes isolated from Sch B pretreated rats. Hepatocytes isolated from Sch B pretreated (a daily dose of 1 mmol/kg for 3 days) rats showed a significant increase (25%) in DTD activity. The increase in DTD activity was associated with the enhanced rate of menadione elimination in the hepatocyte culture. The ensemble of results suggests that the ability of Sch B pretreatment to enhance hepatocellular DTD activity may at least in part be attributed to the protection against menadione hepatotoxicity.
用五味子醇甲（Sch B）对小鼠进行预处理，Sch B是从五味子果实中分离出的一种二苯并环辛二烯衍生物，每日剂量为1 mmol/kg，连续3天，可保护小鼠免受甲萘醌诱导的肝氧化损伤，与甲萘醌中毒对照组相比，血浆丙氨酸转氨酶活性降低（78%）和肝丙二醛水平降低（70%）证明了这一点。为了确定Sch B预处理提供肝保护作用的生化机制，我们检测了从Sch B预处理大鼠分离的肝细胞中DT-黄递酶（DTD）的活性。从Sch B预处理（每日剂量1 mmol/kg，连续3天）大鼠分离的肝细胞显示DTD活性显著增加（25%）。DTD活性的增加与肝细胞培养中甲萘醌消除率的提高有关。这些结果表明，Sch B预处理增强肝细胞DTD活性的能力可能至少部分归因于对甲萘醌肝毒性的保护作用。
