Cannon D M, Winograd N, Ewing A G
Department of Chemistry, Pennsylvania State University, University Park 16802, USA.
Annu Rev Biophys Biomol Struct. 2000;29:239-63. doi: 10.1146/annurev.biophys.29.1.239.
A fundamental perspective can be achieved by targeting single cells for analysis with the goal of deconvoluting complex biological functions. However, single-cell studies have their own difficulties, such as minute volumes and sample amounts. Quantitative chemical analysis of single cells has emerged as a powerful new area in recent years due to several technological advancements. The development of microelectrodes has allowed the measurement of redox-active species as a function of cellular dynamics. This miniaturization trend is also evident in the separation sciences with the application of small column separations to single cells. Desorption ionization methods with mass spectrometric detection have shown single-cell capability owing to numerous technological developments. Finally, fluorescence imaging has also progressed to the point where single-cell dynamics can be probed by native fluorescence utilizing either single or multiple photon excitation. The results of these studies are reviewed with an emphasis on the quantitation of single-cell dynamics.
通过针对单个细胞进行分析以解构复杂生物功能的方式，可以获得一个基本的视角。然而，单细胞研究有其自身的困难，例如微量的体积和样本量。近年来，由于多项技术进步，单细胞的定量化学分析已成为一个强大的新领域。微电极的发展使得能够测量作为细胞动态函数的氧化还原活性物质。这种小型化趋势在分离科学中也很明显，即小柱分离应用于单细胞。由于众多技术发展，带有质谱检测的解吸电离方法已显示出单细胞分析能力。最后，荧光成像也已发展到可以利用单光子或多光子激发通过天然荧光探测单细胞动态的阶段。本文对这些研究结果进行了综述，重点是单细胞动态的定量分析。
