特异性转导噬菌体λ-h80C 1-857 dargF的DNA链的解析
Resolution of the DNA strands of the specialized transducing bacteriophage lambda-h80C 1-857 dargF.
作者信息
Sens D, Eshenbaugh D, James E
出版信息
J Virol. 1975 Jul;16(1):85-93. doi: 10.1128/JVI.16.1.85-93.1975.
Abstract
The DNA strands of lambdoid phages with deletions or substitutions of the guanine plus cytosine-rich region in the left arm are not resolvable by complexing with poly UG followed by centrifugation in CsCl. This work describes a completely general procedure for the strand resolution of these phages by hybridization with fragments of separated strands of the parent phage. In particular, resolution of the DNA strands of the specialized transducing phage lambda-h80C1-857dargF is described, and evidence is presented which indicates that argF is transcribed from the r strand.
摘要
左臂中鸟嘌呤加胞嘧啶丰富区域有缺失或替换的λ样噬菌体的DNA链，通过与聚UG复合然后在CsCl中离心是无法分离的。这项工作描述了一种通过与亲本噬菌体单链片段杂交来完全通用地分离这些噬菌体链的方法。特别描述了特异性转导噬菌体λ-h80C1-857dargF的DNA链的分离，并提供了证据表明argF是从r链转录而来的。
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引用本文的文献
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