噬菌体Mu-1的不对称转录
Asymmetric transcription of bacteriophage Mu-1.
作者信息
Bade E G
出版信息
J Virol. 1972 Dec;10(6):1205-7. doi: 10.1128/JVI.10.6.1205-1207.1972.
Abstract
The deoxyribonucleic acid (DNA) of bacteriophage Mu-1 can be separated into its complementary strands by poly(U,G) binding and equilibrium centrifugation. DNA-ribonucleic acid (RNA) hybridizations in liquid show that more than 98% of "early" phage-specific RNA and over 96% of "late" messenger species bind to the heavy [poly(U,G)-binding] strand of Mu-1 DNA. A small (1.45%) but significant amount of late RNA binds to the light strand. The significance of this RNA fraction is discussed in connection with the peculiar structure of denatured and reannealed Mu-1 DNA.
摘要
噬菌体Mu-1的脱氧核糖核酸（DNA）可通过聚（U，G）结合和平衡离心分离成互补链。液相中的DNA-核糖核酸（RNA）杂交表明，超过98%的“早期”噬菌体特异性RNA和超过96%的“晚期”信使种类与Mu-1 DNA的重链[聚（U，G）结合链]结合。少量（1.45%）但显著量的晚期RNA与轻链结合。结合变性和复性的Mu-1 DNA的特殊结构讨论了该RNA组分的意义。
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