大肠杆菌K-12 argA、argE和argCBH操纵子的体外转录
In vitro transcription of the Escherichia coli K-12 argA, argE, and argCBH operons.
作者信息
Sens D, Natter W, James E
出版信息
J Bacteriol. 1977 May;130(2):642-55. doi: 10.1128/jb.130.2.642-655.1977.
Abstract
Deoxyribonucleic acid isolated from argA and argECBH transducing phages was utilized to study the in vitro synthesis of argA, argE, and argCBH messenger ribonucleic acid. The specific regulation of these operons by the arginine holorepressor was demonstrated, providing evidence that the majority, if not all, of the control of these operons is exercised at the transcriptional level. Data are presented which indicate that the arginine holorepressor functions by binding to the operator region and concomitantly prevents the binding of ribonucleic acid polymerase to the corresponding promoter region.
摘要
从argA和argECBH转导噬菌体中分离出的脱氧核糖核酸被用于研究argA、argE和argCBH信使核糖核酸的体外合成。证实了精氨酸全阻遏物对这些操纵子的特异性调控，这表明这些操纵子的大部分（如果不是全部）控制作用是在转录水平上发挥的。所呈现的数据表明，精氨酸全阻遏物通过与操纵区结合发挥作用，并同时阻止核糖核酸聚合酶与相应的启动子区结合。
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