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特异性转导噬菌体phi80dargF和λh80cI857 dargF的分离与鉴定：核酸内切酶EcoRI和SmaR对精氨酸转导脱氧核糖核酸的特异性切割

Isolation and characterization of the specialized transducing bacteriophages phi80dargF and lambdah80cI857 dargF: specific cleavage of arginine transducing deoxyribonucleic acid by the endonucleases EcoRI and SmaR.

作者信息

James P M, Sens D, Natter W, Moore S K, James E

出版信息

J Bacteriol. 1976 Apr;126(1):487-500. doi: 10.1128/jb.126.1.487-500.1976.

DOI:10.1128/jb.126.1.487-500.1976
PMID:770435
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC233305/
Abstract

The directed transposition of argF to the tonB locus of the Escherichia coli chromosome and the subsequent isolation of the specialized transducing phage phi80dargF is described. The structure of this phage has been has been determined. A hybrid lambdah80cI857dargF phage has been constructed. Deoxyribonucleic acid isolated from these and their parent bacteriophages has been specifically cleaved by the endonucleases EcoRI and SmaR; the unique deoxyribonucleic acid fragments thus obtained have been resolved and analyzed by electrophoresis in agarose gel.

摘要

本文描述了将argF定向转座到大肠杆菌染色体的tonB位点，并随后分离出特异性转导噬菌体phi80dargF的过程。已确定了该噬菌体的结构。构建了一种杂种λh80cI857dargF噬菌体。从这些噬菌体及其亲本噬菌体中分离出的脱氧核糖核酸已被内切酶EcoRI和SmaR特异性切割；由此获得的独特脱氧核糖核酸片段已通过琼脂糖凝胶电泳进行分离和分析。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c884/233305/97115a21716b/jbacter00317-0505-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c884/233305/545485d818b5/jbacter00317-0505-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c884/233305/97115a21716b/jbacter00317-0505-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c884/233305/545485d818b5/jbacter00317-0505-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c884/233305/97115a21716b/jbacter00317-0505-b.jpg

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1
Isolation and characterization of the specialized transducing bacteriophages phi80dargF and lambdah80cI857 dargF: specific cleavage of arginine transducing deoxyribonucleic acid by the endonucleases EcoRI and SmaR.特异性转导噬菌体phi80dargF和λh80cI857 dargF的分离与鉴定：核酸内切酶EcoRI和SmaR对精氨酸转导脱氧核糖核酸的特异性切割
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引用本文的文献

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Molecular characterization of ilvC specialized transducing phages of Escherichia coli K-12.大肠杆菌K-12的ilvC特异性转导噬菌体的分子特征分析

本文引用的文献

1
THE EFFECT OF ACRIDINE DYES ON MATING TYPE FACTORS IN ESCHERICHIA COLI.吖啶染料对大肠杆菌交配型因子的影响
Proc Natl Acad Sci U S A. 1960 Jan;46(1):57-64. doi: 10.1073/pnas.46.1.57.
2
Mutants of Escherichia coli requiring methionine or vitamin B12.需要甲硫氨酸或维生素B12的大肠杆菌突变体。
J Bacteriol. 1950 Jul;60(1):17-28. doi: 10.1128/jb.60.1.17-28.1950.
3
Replica plating and indirect selection of bacterial mutants.细菌突变体的影印培养和间接筛选
Mol Gen Genet. 1983;191(3):347-52. doi: 10.1007/BF00425744.
4
Mapping of restriction sites in the attachment site region of bacteriophage lambda.λ噬菌体附着位点区域限制性酶切位点的图谱绘制
Mol Gen Genet. 1977 Sep 9;154(3):231-48. doi: 10.1007/BF00571278.
5
Cleavage of lambda DNA by a site-specific endonuclease from Serratia marcescens.粘质沙雷氏菌的位点特异性核酸内切酶对λ噬菌体DNA的切割
J Virol. 1976 Sep;19(3):1006-11. doi: 10.1128/JVI.19.3.1006-1011.1976.
6
In vitro transcription of the Escherichia coli K-12 argA, argE, and argCBH operons.大肠杆菌K-12 argA、argE和argCBH操纵子的体外转录
J Bacteriol. 1977 May;130(2):642-55. doi: 10.1128/jb.130.2.642-655.1977.
7
Synthesis of the Escherichia coli K12 isoenzymes of ornithine transcarbamylase, performed in vitro.体外合成大肠杆菌K12鸟氨酸转氨甲酰酶同工酶。
Mol Gen Genet. 1977 Nov 29;157(2):155-65. doi: 10.1007/BF00267393.
8
Transcription of the argF and argI genes of the arginine biosynthetic regulon of Escherichia coli K12, performed in vitro.在体外对大肠杆菌K12精氨酸生物合成调节子的argF和argI基因进行转录。
Mol Gen Genet. 1977 Sep 21;155(1):7-18. doi: 10.1007/BF00268555.
9
Metabolism of arginine-specific messenger ribonucleic acid in Escherichia coli K-12.大肠杆菌K-12中精氨酸特异性信使核糖核酸的代谢
J Bacteriol. 1977 Jul;131(1):214-23. doi: 10.1128/jb.131.1.214-223.1977.
J Bacteriol. 1952 Mar;63(3):399-406. doi: 10.1128/jb.63.3.399-406.1952.
4
Transduction of linked genetic characters of the host by bacteriophage P1.噬菌体P1对宿主连锁遗传性状的转导
Virology. 1955 Jul;1(2):190-206. doi: 10.1016/0042-6822(55)90016-7.
5
Allelic amber and ochre suppressors.等位琥珀型和赭石型抑制基因。
J Mol Biol. 1968 Sep 14;36(2):209-18. doi: 10.1016/0022-2836(68)90376-8.
6
Suppressor-sensitive mutants of coliphage phi-80.大肠杆菌噬菌体phi-80的抑制敏感突变体。
Virology. 1968 Apr;34(4):637-49.
7
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
8
Interaction of prophages at the att80 site with the chromosome of Escherichia coli.原噬菌体在att80位点与大肠杆菌染色体的相互作用。
J Mol Biol. 1966 Jan;15(1):243-55. doi: 10.1016/s0022-2836(66)80224-3.
9
Isolation of the gal repressor.半乳糖阻遏物的分离
Proc Natl Acad Sci U S A. 1971 Aug;68(8):1891-5. doi: 10.1073/pnas.68.8.1891.
10
Mapping the gene determining ornithine transcarbamylase and its operator in Escherichia coli B.绘制大肠杆菌B中决定鸟氨酸转氨甲酰酶及其操纵基因的基因图谱。
J Bacteriol. 1971 Nov;108(2):645-51. doi: 10.1128/jb.108.2.645-651.1971.