Smith J L, Blythe M J, Patterson L T
Poult Sci. 1975 Jan;54(1):183-90. doi: 10.3382/ps.0540183.
A modified skin window system was developed and utilized for examination of the local inflammatory response in the chicken. Data were collected at 2, 4, 6, 8, 12, 24, 28, 32 and 48 hours following wing-web scarification. The first cells observed were heterophils and they remained the predominant type through 32 hours. Mononuclears began infiltrating at a rapid rate of 8 to 12 hours, and by 48 hours they represented slightly more than one-half of the total cells observed. The total number of cells present increased rapidly to a peak at 24 hours, and then declined during the 24 to 48 hour period. The percentage of heterophils and mononuclears showed an inverse relationship throughout the 48 hour period, as appreciable numbers of eosinophils and basophils were not observed. Macrophages were the predominant mononuclear cells. Degranulation of heterophils was extensive in the 2 to 4 hour periods, but was much less common thereafter. As the time after scarification increased, macrophages exhibited; (1) increased cytoplasm to nucleus ratio, (2) increased numbers of phagocytic vacuoles, and (3) a marked tendency to form giant cells. The results obtained in this study were similar to those recorded for mammals, except that the percentage shift toward mononuclears occurred at a faster rate in mammals.
开发并利用了一种改良的皮肤窗系统来检测鸡的局部炎症反应。在翼蹼划痕后2、4、6、8、12、24、28、32和48小时收集数据。观察到的第一批细胞是嗜异性粒细胞，在32小时内它们一直是主要类型。单核细胞在8至12小时开始快速浸润，到48小时时，它们占观察到的总细胞数的略多于一半。存在的细胞总数在24小时迅速增加到峰值，然后在24至48小时期间下降。在整个48小时期间，嗜异性粒细胞和单核细胞的百分比呈反比关系，因为未观察到明显数量的嗜酸性粒细胞和嗜碱性粒细胞。巨噬细胞是主要的单核细胞。嗜异性粒细胞的脱粒在2至4小时期间广泛存在，但此后不太常见。随着划痕后时间的增加，巨噬细胞表现出：（1）细胞质与细胞核的比例增加，（2）吞噬泡的数量增加，以及（3）形成巨细胞的明显趋势。本研究获得的结果与哺乳动物记录的结果相似，只是在哺乳动物中向单核细胞的百分比转变发生得更快。
