Latimer K S, Tang K N, Goodwin M A, Steffens W L, Brown J
Department of Veterinary Pathology, College of Veterinary Medicine, University of Georgia, Athens 30602.
Avian Dis. 1988 Oct-Dec;32(4):760-72.
Leukocyte changes in chickens with turpentine-induced inflammation were investigated sequentially at 0, 6, 12, and 24 hours and at 2, 3, 4, 7, and 14 days. During acute inflammation, significant leukocytosis and heterophilia developed by 6 hours and persisted through 7 days. The peak mean heterophil and leukocyte counts occurred at 12 hours and 3 days, respectively. Left shifts were present at 12 and 24 hours as detected by 100-cell leukocyte differential counts. Heterophil mean nuclear scores documented nuclear hyposegmentation (left shift) during early inflammation and nuclear hypersegmentation (right shift) during convalescence. Mean monocyte and lymphocyte counts peaked at 2 and 3 days, respectively. Basophil and eosinophil counts were erratic. Toxic changes of heterophils were most apparent during intense left shifts and consisted of cell swelling, degranulation, cytoplasmic vacuolation, and cytoplasmic basophilia. Cytoplasmic basophilia was the last aspect of toxic change to resolve. Ultrastructurally, toxic heterophils had intracellular edema, dissolution of granules, retention of ribosomes, nuclear membrane blebs, and decreased heterochromatin density. All inflammation-associated alterations in cell counts and morphology returned to baseline values and appearance by 14 days after turpentine administration.
在0、6、12和24小时以及2、3、4、7和14天对松节油诱导炎症的鸡的白细胞变化进行了连续研究。在急性炎症期间，6小时时出现显著的白细胞增多和嗜异性粒细胞增多，并持续7天。嗜异性粒细胞和白细胞计数的峰值分别出现在12小时和3天。通过100个细胞的白细胞分类计数检测到，12小时和24小时出现核左移。嗜异性粒细胞平均核分数记录了炎症早期的核分叶减少（核左移）和恢复期的核分叶过多（核右移）。单核细胞和淋巴细胞计数的峰值分别出现在2天和3天。嗜碱性粒细胞和嗜酸性粒细胞计数不稳定。嗜异性粒细胞的毒性变化在强烈核左移期间最为明显，包括细胞肿胀、脱颗粒、细胞质空泡化和细胞质嗜碱性。细胞质嗜碱性是毒性变化中最后消失的方面。在超微结构上，毒性嗜异性粒细胞有细胞内水肿、颗粒溶解、核糖体保留、核膜泡和异染色质密度降低。松节油给药后14天，所有与炎症相关的细胞计数和形态变化均恢复到基线值和外观。
