Coudert F, Richard J
Poult Sci. 1975 Jan;54(1):59-63. doi: 10.3382/ps.0540059.
Several methods have been tried in attempts to purify the lymphocytes of chicken blood. The most efficient one is based upon the properties of adhesion of the polymorphonuclears and monocytes. With the use of a column filled with synthetic fibers, held at 37 degrees C., a high rate of lymphocyte purification was obtained. Sedimentation and centrifugation then resulted in the elimination of almost all of the erythrocytes. The final product was homogeneous and the lymphocytes obtained kept the physiological and metabolic properties necessary for their culture.
为了纯化鸡血中的淋巴细胞，人们尝试了几种方法。最有效的一种方法是基于多形核细胞和单核细胞的黏附特性。使用填充有合成纤维的柱子，保持在37摄氏度，可获得较高的淋巴细胞纯化率。然后通过沉降和离心几乎可以去除所有红细胞。最终产物是均匀的，所获得的淋巴细胞保持了其培养所需的生理和代谢特性。
