一种使用全血在体外刺激鸡淋巴细胞的微量方法。
A micromethod for stimulation of chicken lymphocytes in vitro using whole blood.
作者信息
Lassila O, Eskola J, Toivanen P
出版信息
Clin Exp Immunol. 1976 Dec;26(3):641-6.
Abstract
A simple, rapid and reproducible micromethod for determination of in vitro mitogenic responses of chicken peripheral blood lymphocytes is described. The test utilizes 5 mul volumes of heparinized whole blood, 125I-labelled 5-iodo-2'-deoxyuridine with 5-fluoro-2'-deoxyuridine in place of [3H]thymidine and a multiple cell-culture harvester. The method permits a close follow-up of the mitogenic responses in the chicken.
摘要
本文描述了一种简单、快速且可重复的微量方法，用于测定鸡外周血淋巴细胞的体外促有丝分裂反应。该试验使用5微升肝素化全血、用5-氟-2'-脱氧尿苷代替[3H]胸腺嘧啶核苷的125I标记的5-碘-2'-脱氧尿苷以及多细胞培养收获仪。该方法能够密切跟踪鸡体内的促有丝分裂反应。
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A micromethod for stimulation of chicken lymphocytes in vitro using whole blood.一种使用全血在体外刺激鸡淋巴细胞的微量方法。
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Nature. 1968 Oct 19;220(5164):293-5. doi: 10.1038/220293a0.
2
In vitro studies of chicken lymphoid cells. 1. Phytohaemagglutinin induced DNA, RNA and protein synthesis in spleen cells from control-irradiated and bursectomized-irradiated chickens.鸡淋巴细胞的体外研究。1. 植物血凝素诱导对照照射鸡和法氏囊切除照射鸡脾脏细胞中的DNA、RNA和蛋白质合成。
Acta Pathol Microbiol Scand B Microbiol Immunol. 1970;78(5):632-40.
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