De Mulder P H, Wessels J M, Rosenbrand D A, Smeulders J B, Wagener D J, Haanen C
J Immunol Methods. 1981;47(1):31-8. doi: 10.1016/0022-1759(81)90254-4.
Continuous monitoring of cell light scatter during counterflow centrifugation of a mononuclear cell suspension allows counting and size recognition of the cell types elutriated. With this method an optimal separation point between monocytes and lymphocytes, determined for each individual donor, may be established. With a constant flow of 15 ml/min this separation point is found at centrifugal velocities ranging from 2348 to 2444 rpm (n = 10). From 50 ml venous blood, 84.1% +/- 4.1% (15.7 +/- 8.6 x 10(6)) of all elutriated monocytes, with a purity of 92.4% +/- 1.4%, is collected in a volume of 50 +/- 1 ml. In the same run, 92% +/- 4.3% of the lymphocytes is gathered in one fraction with a purity of 98.9% +/- 0.7%. After counterflow centrifugation, 91.6 +/- 10.5% of the cells loaded is recovered; viability exceeds 98%.
在单核细胞悬液逆流离心过程中持续监测细胞光散射，可对洗脱的细胞类型进行计数和大小识别。通过这种方法，可以为每个个体供体确定单核细胞和淋巴细胞之间的最佳分离点。当流速恒定为15毫升/分钟时，在2348至2444转/分钟的离心速度范围内可找到该分离点（n = 10）。从50毫升静脉血中，收集到的所有洗脱单核细胞的84.1%±4.1%（15.7±8.6×10⁶），纯度为92.4%±1.4%，收集体积为50±1毫升。在同一轮操作中，92%±4.3%的淋巴细胞聚集在一个组分中，纯度为98.9%±0.7%。逆流离心后，加载细胞的回收率为91.6±10.5%；活力超过98%。
