Gottesman S, Gottesman M
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2188-92. doi: 10.1073/pnas.72.6.2188.
A cell-free system that promotes the excision of prophage lambda DNA has been established. The substrate for the reaction is phage DNA carrying two attachment sites, which, in vivo, undergoes intramolecular recombination between these sites. The in vitro recombination system is efficient; 25-35% of the substrate DNA undergoes recombination in 30 min. There is an absolute requirement for ATP; Mg++ and spermidine are stimulatory. RNA does not appear to be involved, nor can a role for DNA synthesis be demonstrated.
已经建立了一种促进λ原噬菌体DNA切除的无细胞系统。该反应的底物是携带两个附着位点的噬菌体DNA，在体内，这些位点之间会发生分子内重组。体外重组系统效率很高；30分钟内25%-35%的底物DNA会发生重组。绝对需要ATP；Mg++和亚精胺有促进作用。RNA似乎不参与其中，也无法证明DNA合成有作用。
