Doskocil J, Forstová J, Hauserová E
Mol Gen Genet. 1981;182(1):125-32. doi: 10.1007/BF00422778.
Restriction maps of genomes of the lytic form and diverse temperate mutants of phage theta of Bacillus licheniformis were constructed. Most temperate mutants produced fragmentation patterns identical to that of the parent lytic form, theta c: in other mutants the only detectable change in the map was the deletion of a Bg/II restriction endonuclease site at 46.5% genome length. In the genomes of two other temperate mutants, theta 1 and theta 2, the central part of the genome was replaced by a piece of DNA of equal length, but with a different distribution of restriction sites; the maps of the two mutants failed to reveal any similarity in the location of restriction sites in the inserted DNA. It seems that any alteration comprising the locus around the coordinate 46.5% of the theta c genome, brings about a transition from the lytic to temperate phenotype, indicating the position of a regulatory gene responsible for positive control of phage replication.
构建了地衣芽孢杆菌噬菌体θ的裂解型基因组和多种温和突变体的限制图谱。大多数温和突变体产生的片段化模式与亲本裂解型θc相同：在其他突变体中，图谱中唯一可检测到的变化是在基因组长度46.5%处的一个Bg/II限制内切酶位点缺失。在另外两个温和突变体θ1和θ2的基因组中，基因组的中心部分被一段等长但限制位点分布不同的DNA所取代；这两个突变体的图谱未能揭示插入DNA中限制位点位置的任何相似性。似乎任何包含θc基因组坐标46.5%周围位点的改变都会导致从裂解型到温和型表型的转变，这表明了一个负责噬菌体复制正调控的调节基因的位置。
