DNA促旋酶：一种将超螺旋引入DNA的酶。
DNA gyrase: an enzyme that introduces superhelical turns into DNA.
作者信息
Gellert M, Mizuuchi K, O'Dea M H, Nash H A
出版信息
Proc Natl Acad Sci U S A. 1976 Nov;73(11):3872-6. doi: 10.1073/pnas.73.11.3872.
Abstract
Relaxed closed-circular DNA is converted to negatively supercoiled DNA by DNA gyrase. This enzyme has been purified from Escherichia coli cells. The reaction requires ATP and Mg++ and is stimulated by spermidine. The enzyme acts equally well on relaxed closed-circular colicin E1, phage lambda, and simian virus 40 DNA. The final superhelix density of the DNA can be considerably greater than that found in intracellularly supercoiled DNA.
摘要
松弛的闭环DNA被DNA促旋酶转化为负超螺旋DNA。这种酶已从大肠杆菌细胞中纯化出来。该反应需要ATP和Mg++，并受到亚精胺的刺激。该酶对松弛的闭环大肠杆菌素E1、噬菌体λ和猴病毒40 DNA的作用效果相同。DNA的最终超螺旋密度可能比细胞内超螺旋DNA中的超螺旋密度大得多。
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