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生长条件对大肠杆菌中DNA与膜附着的影响。

Effect of growth conditions on DNA-membrane attachment in Escherichia coli.

作者信息

Parker D L, Glaser D A

出版信息

Proc Natl Acad Sci U S A. 1975 Jun;72(6):2446-50. doi: 10.1073/pnas.72.6.2446.

Abstract

DNA-membrane attachment in cultures of E. coli B/r is found to depend on the pattern of DNA synthesis, (a) When the number of replication points increases, the fraction of the DNA that is membrane bound also increases. The relationship between the amount of DNA-membrane attachment and the number of replication points is most consistent with attachment of all replication points and attachment of at least one other chromosomal site; it is inconsistent with attachment of the replication points only, the chromosomal origin only, or the chromosomal terminus only. (b) When repair DNA synthesis is stimulated by irradiation with ultraviolet light, the DNA-membrane attachment remains unchanged.

摘要

大肠杆菌B/r培养物中的DNA与膜的附着被发现取决于DNA合成模式,(a)当复制点数量增加时,与膜结合的DNA比例也会增加。DNA与膜的附着量与复制点数量之间的关系最符合所有复制点以及至少一个其他染色体位点的附着情况;这与仅复制点、仅染色体原点或仅染色体终点的附着情况不一致。(b)当用紫外线照射刺激修复DNA合成时,DNA与膜的附着保持不变。

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