Restriction enzyme cleavage of DNA resulting from gently lysed Escherichia coli.

When E. coli or lambda infected E. coli are gently lysed the DNA is released as a very fast sedimenting species that is presumably bound to membrane material. If this complex is now subjected to restriction enzyme cleavage, only a minor fraction of the fast sedimenting DNA remains and this is found, after purification, to be enriched for branched molecules.