Kim H M, Shin H Y, Lim K H, Ryu S T, Shin T Y, Chae H J, Kim H R, Lyu Y S, An N H, Lim K S
College of Pharmacy, Center of Oriental Medicinal Science, Wonkwang University, Iksan, Chonbuk, South Korea.
Immunopharmacol Immunotoxicol. 2000 Aug;22(3):519-30. doi: 10.3109/08923970009026009.
Substance P (SP) can stimulate production of tumor necrosis factor-alpha (TNF-alpha) from astrocytes stimulated with lipopolysaccharide (LPS). The objective of the current study was to determine the effect of Taraxacum officinale (TO) on the production of TNF-alpha from primary cultures of rat astrocytes. TO (100 and 1000 microg/ml) significantly inhibited the TNF-alpha production by astrocytes stimulated with LPS and SP. Interleukin-1 (IL-1) has been shown to elevate TNF-alpha production from LPS-stimulated astrocytes while having no effect on astrocytes in the absence of LPS. We therefore examined whether IL-1 mediated inhibition of TNF-alpha production from primary astrocytes by TO. Treatment of TO (100 and 1000 microg/ml) to astrocytes stimulated with both LPS and SP decreased IL-1 production significantly. Moreover, the production of TNF-alpha by LPS and SP in astrocytes was progressively inhibited with increasing amount of IL-1 neutralizing antibody. Our results suggest that TO may inhibit TNF-alpha production by inhibiting IL-1 production and that TO has an antiinflammatory activity in the central nervous system.
P物质（SP）可刺激经脂多糖（LPS）刺激的星形胶质细胞产生肿瘤坏死因子-α（TNF-α）。本研究的目的是确定蒲公英（TO）对大鼠原代星形胶质细胞产生TNF-α的影响。TO（100和1000微克/毫升）显著抑制了经LPS和SP刺激的星形胶质细胞产生TNF-α。白细胞介素-1（IL-1）已被证明可提高经LPS刺激的星形胶质细胞产生TNF-α的水平，而在无LPS的情况下对星形胶质细胞无影响。因此，我们研究了IL-1是否介导TO对原代星形胶质细胞产生TNF-α的抑制作用。用TO（100和1000微克/毫升）处理经LPS和SP刺激的星形胶质细胞可显著降低IL-1的产生。此外，随着IL-1中和抗体量的增加，星形胶质细胞中LPS和SP诱导的TNF-α产生逐渐受到抑制。我们的结果表明，TO可能通过抑制IL-1的产生来抑制TNF-α的产生，并且TO在中枢神经系统中具有抗炎活性。
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