Kim H M, Shin T Y
Department of Oriental Pharmacy, College of Pharmacy, Center of Oriental Medicinal Science, Wonkwang University, Iksan, South Korea.
Immunopharmacol Immunotoxicol. 2001 Feb;23(1):97-106. doi: 10.1081/iph-100102571.
Substance P (SP) can stimulate secretion of tumor necrosis factor-alpha (TNF-alpha) from astrocytes stimulated with lipopolysaccharide (LPS). In this study, we have examined whether an aqueous extract of Chilbokeum inhibits secretion of TNF-alpha from primary cultures of rat astrocytes. Chilbokeum (10 microg/ml) significantly inhibited the TNF-alpha secretion by astrocytes stimulated with LPS and SP. Interleukin-1 (IL-1) has been shown to elevate TNF-alpha secretion from LPS-stimulated astrocytes while having no effect on astrocytes in the absence of LPS. We therefore examined whether IL-1 mediated inhibition of TNF-alpha secretion from primary astrocytes by Chilbokeum. Treatment of Chilbokeum (10 and 100 microg/ml) to astrocytes stimulated with both LPS and SP decreased IL-1 secretion significantly. Moreover, the secretion of TNF-alpha by LPS and SP in astrocytes was progressively inhibited with increasing amount of IL-1 neutralizing antibody. Our results suggest that Chilbokeum may inhibit TNF-alpha secretion by inhibiting IL-1 secretion and that Chilbokeum has an antiinflammatory activity in the central nervous system.
P物质（SP）可刺激经脂多糖（LPS）刺激的星形胶质细胞分泌肿瘤坏死因子-α（TNF-α）。在本研究中，我们检测了赤膊金水溶液提取物是否能抑制大鼠星形胶质细胞原代培养物中TNF-α的分泌。赤膊金（10微克/毫升）显著抑制了经LPS和SP刺激的星形胶质细胞分泌TNF-α。白细胞介素-1（IL-1）已被证明可提高经LPS刺激的星形胶质细胞的TNF-α分泌，而在无LPS的情况下对星形胶质细胞无影响。因此，我们检测了IL-1是否介导了赤膊金对原代星形胶质细胞TNF-α分泌的抑制作用。用赤膊金（10和100微克/毫升）处理经LPS和SP刺激的星形胶质细胞可显著降低IL-1的分泌。此外，随着IL-1中和抗体量的增加，LPS和SP刺激的星形胶质细胞中TNF-α的分泌逐渐受到抑制。我们的结果表明，赤膊金可能通过抑制IL-1分泌来抑制TNF-α分泌，并且赤膊金在中枢神经系统中具有抗炎活性。
