Kim H M, An C S, Jung K Y, Choo Y K, Park J K, Nam S Y
Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Iksan, Chonbuk, 570-749, South Korea.
Pharmacol Res. 1999 Aug;40(2):171-6. doi: 10.1006/phrs.1999.0504.
We investigated whether an aqueous extract of Rehmannia glutinosa steamed root (RGAE) inhibits secretion of tumour necrosis factor-alpha (TNF-alpha) and interleukin-1 (IL-1) from primary cultures of mouse astrocytes. RGAE dose-dependently inhibited the TNF-alpha secretion by astrocytes stimulated with substance P (SP) and lipopolysaccharide (LPS). IL-1 has been shown to elevate TNF-alpha secretion from LPS-stimulated astrocytes while having no effect on astrocytes in the absence of LPS. We therefore investigated whether IL-1 mediated inhibition of TNF-alpha secretion from astrocytes by RGAE. Treatment of RGAE to astrocytes stimulated with both LPS+SP decreased IL-1 secretion. Moreover, incubation of astrocytes with IL-1 antibody abolished the synergistic cooperative effect of LPS+SP. These results suggest that RGAE may inhibits TNF-alpha secretion by inhibiting IL-1 secretion and that RGAE has an anti-inflammatory activity in the central nervous system curing some pathological disease states.
我们研究了熟地黄水提取物（RGAE）是否能抑制小鼠星形胶质细胞原代培养物中肿瘤坏死因子-α（TNF-α）和白细胞介素-1（IL-1）的分泌。RGAE剂量依赖性地抑制了由P物质（SP）和脂多糖（LPS）刺激的星形胶质细胞分泌TNF-α。IL-1已被证明能提高LPS刺激的星形胶质细胞分泌TNF-α，而在无LPS的情况下对星形胶质细胞没有影响。因此，我们研究了IL-1是否介导RGAE对星形胶质细胞分泌TNF-α的抑制作用。用RGAE处理LPS+SP刺激的星形胶质细胞可降低IL-1分泌。此外，用IL-1抗体孵育星形胶质细胞消除了LPS+SP的协同合作效应。这些结果表明，RGAE可能通过抑制IL-1分泌来抑制TNF-α分泌，并且RGAE在中枢神经系统中治疗某些病理疾病状态时具有抗炎活性。
