通过在大肠杆菌中进行亚克隆培养从未分级的海胆DNA中分离组蛋白基因。
Isolation of histone genes from unfractionated sea urchin DNA by subculture cloning in E. coli.
作者信息
Kedes L H, Chang A C, Houseman D, Cohen S N
出版信息
Nature. 1975 Jun 12;255(5509):533-8. doi: 10.1038/255533a0.
DOI:10.1038/255533a0
PMID:1095932
Abstract
Histone genes have been cloned selectively in Escherichia coli directly from unfractionated sea urchin DNA using labelled mRNA probe. The cloning method used is potentially applicable for isolating any genetic sequence for which a probe is available. The several eukaryotic gene fragments introduced into bacteria by this method collectively contain all or nearly all of the DNA sequences represented in histone mRNA.
摘要
利用标记的信使核糖核酸（mRNA）探针，已直接从未分级的海胆DNA中在大肠杆菌中选择性克隆了组蛋白基因。所使用的克隆方法有可能适用于分离任何有可用探针的遗传序列。通过这种方法引入细菌的几个真核基因片段共同包含了组蛋白mRNA中所代表的全部或几乎全部DNA序列。
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