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1
The NosX and NirX proteins of Paracoccus denitrificans are functional homologues: their role in maturation of nitrous oxide reductase.反硝化副球菌的NosX和NirX蛋白是功能同源物：它们在一氧化二氮还原酶成熟中的作用。
J Bacteriol. 2000 Sep;182(18):5211-7. doi: 10.1128/JB.182.18.5211-5217.2000.
2
NosX function connects to nitrous oxide (N2O) reduction by affecting the Cu(Z) center of NosZ and its activity in vivo.NosX的功能通过影响NosZ的铜（Z）中心及其体内活性与一氧化二氮（N2O）还原相关。
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is essential for whole-cell NO reduction in but not for assembly of copper centres of nitrous oxide reductase.对于完整细胞中 NO 的还原是必需的，但对于亚硝酸盐还原酶铜中心的组装则不是必需的。
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Transcription regulation of the nir gene cluster encoding nitrite reductase of Paracoccus denitrificans involves NNR and NirI, a novel type of membrane protein.编码反硝化副球菌亚硝酸还原酶的nir基因簇的转录调控涉及NNR和NirI，NirI是一种新型膜蛋白。
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Expression of nitrous oxide reductase in Paracoccus denitrificans is regulated by oxygen and nitric oxide through FnrP and NNR.亚硝化单胞菌中一氧化二氮还原酶的表达受 FnrP 和 NNR 通过氧和一氧化氮的调节。
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Anaerobic growth of Paracoccus denitrificans requires cobalamin: characterization of cobK and cobJ genes.反硝化副球菌的厌氧生长需要钴胺素：cobK和cobJ基因的特性分析
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The nos (nitrous oxide reductase) gene cluster from the soil bacterium Achromobacter cycloclastes: cloning, sequence analysis, and expression.来自土壤细菌解环无色杆菌的一氧化二氮还原酶（nos）基因簇：克隆、序列分析及表达
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Plant-Pathogenic Phylotypes Evolved Divergent Respiratory Strategies and Behaviors To Thrive in Xylem.植物病原物 基因型进化出不同的呼吸策略和行为以在木质部中茁壮成长。
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is essential for whole-cell NO reduction in but not for assembly of copper centres of nitrous oxide reductase.对于完整细胞中 NO 的还原是必需的，但对于亚硝酸盐还原酶铜中心的组装则不是必需的。
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NosL is a dedicated copper chaperone for assembly of the Cu center of nitrous oxide reductase.NosL是一种专门用于一氧化二氮还原酶铜中心组装的铜伴侣蛋白。
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7
Mechanisms of copper homeostasis in bacteria.细菌中铜稳态的机制。
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8
Alternative pyrimidine biosynthesis protein ApbE is a flavin transferase catalyzing covalent attachment of FMN to a threonine residue in bacterial flavoproteins.嘧啶生物合成替代蛋白 ApbE 是一种黄素转移酶，可催化 FMN 与细菌黄素蛋白中的苏氨酸残基形成共价键。
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9
Expression of nitrous oxide reductase in Paracoccus denitrificans is regulated by oxygen and nitric oxide through FnrP and NNR.亚硝化单胞菌中一氧化二氮还原酶的表达受 FnrP 和 NNR 通过氧和一氧化氮的调节。
Microbiology (Reading). 2012 Mar;158(Pt 3):826-834. doi: 10.1099/mic.0.054148-0. Epub 2011 Dec 15.
10
Role of a nosX homolog in Streptococcus gordonii in aerobic growth and biofilm formation.nosX 同源物在戈登氏链球菌需氧生长和生物膜形成中的作用
J Bacteriol. 2004 Dec;186(24):8193-206. doi: 10.1128/JB.186.24.8193-8206.2004.

本文引用的文献

1
Transcription regulation of the nir gene cluster encoding nitrite reductase of Paracoccus denitrificans involves NNR and NirI, a novel type of membrane protein.编码反硝化副球菌亚硝酸还原酶的nir基因簇的转录调控涉及NNR和NirI，NirI是一种新型膜蛋白。
Mol Microbiol. 1999 Oct;34(1):24-36. doi: 10.1046/j.1365-2958.1999.01563.x.
2
Nitric oxide is a signal for NNR-mediated transcription activation in Paracoccus denitrificans.一氧化氮是反硝化副球菌中NNR介导的转录激活的信号。
J Bacteriol. 1999 Jul;181(13):4129-32. doi: 10.1128/JB.181.13.4129-4132.1999.
3
A re-evaluation of the taxonomy of Paracoccus denitrificans and a proposal for the combination Paracoccus pantotrophus comb. nov.反硝化副球菌分类学的重新评估及泛养副球菌组合新名称的提议
Int J Syst Bacteriol. 1999 Apr;49 Pt 2:645-51. doi: 10.1099/00207713-49-2-645.
4
The nos (nitrous oxide reductase) gene cluster from the soil bacterium Achromobacter cycloclastes: cloning, sequence analysis, and expression.来自土壤细菌解环无色杆菌的一氧化二氮还原酶（nos）基因簇：克隆、序列分析及表达
J Inorg Biochem. 1998 Jul;70(3-4):155-69. doi: 10.1016/s0162-0134(98)10001-6.
5
Overlapping functions of components of a bacterial Sec-independent protein export pathway.细菌Sec非依赖型蛋白质输出途径各组分的重叠功能。
EMBO J. 1998 Jul 1;17(13):3640-50. doi: 10.1093/emboj/17.13.3640.
6
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.CLUSTAL_X 窗口界面：借助质量分析工具的多序列比对灵活策略。
Nucleic Acids Res. 1997 Dec 15;25(24):4876-82. doi: 10.1093/nar/25.24.4876.
7
A new nos gene downstream from nosDFY is essential for dissimilatory reduction of nitrous oxide by Rhizobium (Sinorhizobium) meliloti.位于nosDFY下游的一个新的nos基因对于苜蓿根瘤菌（中华根瘤菌）异化还原一氧化二氮至关重要。
Microbiology (Reading). 1997 Aug;143 ( Pt 8):2817-2824. doi: 10.1099/00221287-143-8-2817.
8
Lack of copper insertion into unprocessed cytoplasmic nitrous oxide reductase generated by an R20D substitution in the arginine consensus motif of the signal peptide.信号肽精氨酸共有基序中R20D取代产生的未加工细胞质一氧化二氮还原酶缺乏铜插入。
Biochim Biophys Acta. 1997 Apr 11;1319(2-3):311-8. doi: 10.1016/s0005-2728(96)00174-0.
9
Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites.原核生物和真核生物信号肽的鉴定及其切割位点的预测。
Protein Eng. 1997 Jan;10(1):1-6. doi: 10.1093/protein/10.1.1.
10
The PROSITE database, its status in 1997.PROSITE数据库及其1997年的状况。
Nucleic Acids Res. 1997 Jan 1;25(1):217-21. doi: 10.1093/nar/25.1.217.

反硝化副球菌的NosX和NirX蛋白是功能同源物：它们在一氧化二氮还原酶成熟中的作用。

The NosX and NirX proteins of Paracoccus denitrificans are functional homologues: their role in maturation of nitrous oxide reductase.

作者信息

Saunders N F, Hornberg J J, Reijnders W N, Westerhoff H V, de Vries S, van Spanning R J

机构信息

Department of Molecular Cell Physiology, Faculty of Biology, BioCentrum Amsterdam, Vrije Universiteit, Amsterdam, The Netherlands.

出版信息

J Bacteriol. 2000 Sep;182(18):5211-7. doi: 10.1128/JB.182.18.5211-5217.2000.

DOI:10.1128/JB.182.18.5211-5217.2000
PMID:10960107
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC94671/
Abstract

The nos (nitrous oxide reductase) operon of Paracoccus denitrificans contains a nosX gene homologous to those found in the nos operons of other denitrifiers. NosX is also homologous to NirX, which is so far unique to P. denitrificans. Single mutations of these genes did not result in any apparent phenotype, but a double nosX nirX mutant was unable to reduce nitrous oxide. Promoter-lacZ assays and immunoblotting against nitrous oxide reductase showed that the defect was not due to failure of expression of nosZ, the structural gene for nitrous oxide reductase. Electron paramagnetic resonance spectroscopy showed that nitrous oxide reductase in cells of the double mutant lacked the Cu(A) center. A twin-arginine motif in both NosX and NirX suggests that the NosX proteins are exported to the periplasm via the TAT translocon.

摘要

反硝化副球菌的nos（一氧化二氮还原酶）操纵子包含一个nosX基因，该基因与在其他反硝化菌的nos操纵子中发现的基因同源。NosX也与NirX同源，而NirX是迄今为止反硝化副球菌所特有的。这些基因的单突变并未导致任何明显的表型，但nosX nirX双突变体无法还原一氧化二氮。启动子-lacZ分析以及针对一氧化二氮还原酶的免疫印迹表明，该缺陷并非由于一氧化二氮还原酶的结构基因nosZ表达失败所致。电子顺磁共振光谱显示，双突变体细胞中的一氧化二氮还原酶缺乏Cu(A)中心。NosX和NirX中的双精氨酸基序表明，NosX蛋白通过TAT转位酶转运到周质中。