Rainey F A, Kelly D P, Stackebrandt E, Burghardt J, Hiraishi A, Katayama Y, Wood A P
Department of Biological Sciences, Louisiana State University, Baton Rouge 70803, USA.
Int J Syst Bacteriol. 1999 Apr;49 Pt 2:645-51. doi: 10.1099/00207713-49-2-645.
Comparison of both 16S rRNA coding sequences and DNA-DNA hybridization of ten strains of alpha-subclass of Proteobacteria currently classified as strains of Paracoccus denitrificans has shown that they fall into two groups which are distinct from each other at the species level. Comparison with published data on the cytochrome c profiles and other 16S rRNA coding sequences in the literature has confirmed these observations and enabled several other strains also to be assigned to these two groups. Group A comprises strains ATCC 17741T (the type strain of P. denitrificans), LMD 22.21T, DSM 413T, ATCC 19367, ATCC 13543, DSM 1404, DSM 1405, Pd 1222 (a genetic modification of DSM 413T) and NCIMB 8944. Group B comprises ATCC 35512T (the original type strain of Thiosphaera pantotropha), LMD 82.5T, LMD 92.63, DSM 65, LMG 4218, IAM 12479, JCM 6892, DSM 11072, DSM 11073 and DSM 11104. In light of these findings, it is proposed that: (1) strains of group A are retained as P. denitrificans, with ATCC 17741T as the type strain of the type species; and (2) all strains of group B are assigned to the new species combination Paracoccus pantotrophus comb. nov., with strain ATCC 35512T as the type strain. Comparative 16S rRNA sequence analysis and DNA-DNA hybridization of strains of Paracoccus versutus confirm that this species is distinct from both P. denitrificans and P. pantotrophus, but that its nearest phylogenetic neighbour is P. pantotrophus.
对目前归类为反硝化副球菌菌株的10株变形杆菌α-亚类菌株的16S rRNA编码序列和DNA-DNA杂交进行比较，结果表明它们分为两组，在种水平上彼此不同。与文献中已发表的关于细胞色素c谱和其他16S rRNA编码序列的数据进行比较，证实了这些观察结果，并使其他几个菌株也能归入这两组。A组包括菌株ATCC 17741T（反硝化副球菌模式菌株）、LMD 22.21T、DSM 413T、ATCC 19367、ATCC 13543、DSM 1404、DSM 1405、Pd 1222（DSM 413T的基因改造菌株）和NCIMB 8944。B组包括ATCC 35512T（泛养硫球菌原始模式菌株）、LMD 82.5T、LMD 92.63、DSM 65、LMG 4218、IAM 12479、JCM 6892、DSM 11072、DSM 11073和DSM 11104。根据这些发现，建议：（1）将A组菌株保留为反硝化副球菌，以ATCC 17741T作为模式种的模式菌株；（2）将B组所有菌株归入新的种组合泛养副球菌Paracoccus pantotrophus comb. nov.，以菌株ATCC 35512T作为模式菌株。对敏捷副球菌菌株的16S rRNA序列分析和DNA-DNA杂交比较证实，该种与反硝化副球菌和泛养副球菌均不同，但与其最近的系统发育邻种是泛养副球菌。
