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基于比较系统发育分析,将杯状病毒科重新分类为不同的属,并将戊型肝炎病毒排除出该科。

Reclassification of the Caliciviridae into distinct genera and exclusion of hepatitis E virus from the family on the basis of comparative phylogenetic analysis.

作者信息

Berke T, Matson D O

机构信息

Center for Pediatric Research, Children's Hospital of The King's Daughters, Eastern Virginia Medical School, Norfolk, USA.

出版信息

Arch Virol. 2000;145(7):1421-36. doi: 10.1007/s007050070099.

Abstract

Caliciviridae and Picornaviridae belong to the same subphylum and genera within Picornaviridae are well characterized. Until 1998, Caliciviridae included one genus Calicivirus, containing strains with distinct structural and genomic features. Phylogenetic analyses of capsid genes revealed five clusters within Caliciviridae corresponding to differences in genome organization. In order to determine to what taxonomic level these clusters correspond, genomic sequences of caliciviruses, picornavirus prototypes, and two togavirus strains were analyzed. Distance and maximum likelihood methods were used to estimate the phylogenetic relationships among strains. Analysis of the capsid gene revealed separation of five main clusters (Norwalk-like, and Sapporo-like human caliciviruses, hepatitis E virus, vesicular exanthem of swine-like, and lapine caliciviruses) and distances corresponding to those observed among picornavirus genera. Utilizing more conserved (presumed helicase and polymerase) regions for the analyses, only major groups of caliciviruses were separated with confidence, with distances also comparable to those separating picornavirus genera. Analysis in these regions that included togavirus sequences moved HEV strains out of the calicivirus cluster. Our findings support the reclassification of caliciviruses into four genera. The phylogenetic position of hepatitis E virus, by analysis of non-structural genes, is outside of the caliciviruses, in an uncertain taxonomic position.

摘要

杯状病毒科和小核糖核酸病毒科属于同一亚门,小核糖核酸病毒科内的属已得到充分描述。直到1998年,杯状病毒科包括一个杯状病毒属,其中包含具有不同结构和基因组特征的毒株。衣壳基因的系统发育分析显示,杯状病毒科内有五个簇,对应于基因组组织的差异。为了确定这些簇对应于何种分类水平,对杯状病毒、小核糖核酸病毒原型和两种披膜病毒毒株的基因组序列进行了分析。采用距离法和最大似然法估计毒株间的系统发育关系。衣壳基因分析显示五个主要簇(诺如病毒样和札幌病毒样人杯状病毒、戊型肝炎病毒、猪水疱性疹样病毒和兔杯状病毒)的分离以及与小核糖核酸病毒属间观察到的距离相对应。利用更保守的(假定的解旋酶和聚合酶)区域进行分析,只有杯状病毒的主要类群能够可靠地分离,其距离也与区分小核糖核酸病毒属的距离相当。在包括披膜病毒序列的这些区域进行分析,使戊型肝炎病毒毒株移出了杯状病毒簇。我们的研究结果支持将杯状病毒重新分类为四个属。通过对非结构基因的分析,戊型肝炎病毒的系统发育位置在杯状病毒之外,处于不确定的分类位置。

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