L'Homme Yvan, Sansregret Raphaël, Plante-Fortier Etienne, Lamontagne Anne-Marie, Ouardani Mourad, Lacroix Geneviève, Simard Carole
St-Hyacinthe Laboratory, Canadian Food Inspection Agency, 3400 Blvd Casavant West, St-Hyacinthe, QC J2S 8E3, Canada.
Virus Genes. 2009 Aug;39(1):66-75. doi: 10.1007/s11262-009-0360-3. Epub 2009 Apr 26.
This study reports the molecular characterization of novel caliciviruses, the St-Valérien-like viruses, which were isolated from pig feces in the province of Quebec, Canada between 2005 and 2007. The genomes of St-Valérien-like viruses contain 6409 nucleotides and include two main open reading frames (ORFs). ORF1 encodes the non structural (NS) polyprotein and the major capsid protein (VP1) while ORF2 encodes the putative basic minor capsid protein. Typical conserved amino acid motifs predict a gene order reminiscent of calicivirus genomes. Phylogenetic, pairwise homology, and distance analyses performed on complete genomic sequences and partial amino acid sequences from the NTPase, polymerase, and major capsid protein segregated the St-Valérien-like viruses in a unique cluster sharing a common root with the Tulane virus and the noroviruses. Based on the genomic analyses presented, the St-Valérien-like viruses are members of a new genus of Caliciviridae for which we propose the name Valovirus.
本研究报告了新型杯状病毒——圣瓦莱里安样病毒的分子特征，这些病毒于2005年至2007年期间从加拿大魁北克省的猪粪便中分离出来。圣瓦莱里安样病毒的基因组包含6409个核苷酸，包括两个主要的开放阅读框（ORF）。ORF1编码非结构（NS）多聚蛋白和主要衣壳蛋白（VP1），而ORF2编码假定的碱性次要衣壳蛋白。典型的保守氨基酸基序预测了一种让人联想到杯状病毒基因组的基因顺序。对来自NTPase、聚合酶和主要衣壳蛋白的完整基因组序列和部分氨基酸序列进行的系统发育、成对同源性和距离分析，将圣瓦莱里安样病毒归为一个独特的簇，该簇与图兰病毒和诺如病毒有着共同的起源。基于所呈现的基因组分析，圣瓦莱里安样病毒是杯状病毒科一个新属的成员，我们提议将其命名为Valovirus。
