乳酸乳球菌作为用于高水平双乙酰生产的细胞工厂。
Lactococcus lactis as a cell factory for high-level diacetyl production.
作者信息
Hugenholtz J, Kleerebezem M, Starrenburg M, Delcour J, de Vos W, Hols P
机构信息
Wageningen Centre for Food Sciences and NIZO Food Research, 6710 BA Ede, The Netherlands.
出版信息
Appl Environ Microbiol. 2000 Sep;66(9):4112-4. doi: 10.1128/AEM.66.9.4112-4114.2000.
Abstract
We report the engineering of Lactococcus lactis for the efficient conversion of sugar into diacetyl by combining NADH-oxidase overproduction and alpha-acetolactate decarboxylase inactivation. Eighty percent of the carbon flux was found to be rerouted via alpha-acetolactate to the production of diacetyl by preloading the cells with NADH-oxidase before their use as a cell factory.
摘要
我们报道了通过结合过量表达NADH氧化酶和使α-乙酰乳酸脱羧酶失活，对乳酸乳球菌进行工程改造，以实现将糖高效转化为双乙酰。在将细胞用作细胞工厂之前，通过用NADH氧化酶预加载细胞，发现80%的碳通量通过α-乙酰乳酸重新导向双乙酰的生产。
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