通过代谢工程在乳酸乳球菌中高效生产乙醛
High-level acetaldehyde production in Lactococcus lactis by metabolic engineering.
作者信息
Bongers Roger S, Hoefnagel Marcel H N, Kleerebezem Michiel
机构信息
NIZO food research, Department of Flavour and Natural Ingredients, P.O. Box 20, 6710 BA Ede, The Netherlands.
出版信息
Appl Environ Microbiol. 2005 Feb;71(2):1109-13. doi: 10.1128/AEM.71.2.1109-1113.2005.
Abstract
Efficient conversion of glucose to acetaldehyde is achieved by nisin-controlled overexpression of Zymomonas mobilis pyruvate decarboxylase (pdc) and Lactococcus lactis NADH oxidase (nox) in L. lactis. In resting cells, almost 50% of the glucose consumed could be redirected towards acetaldehyde by combined overexpression of pdc and nox under anaerobic conditions.
摘要
通过在乳酸乳球菌中由乳链菌肽控制运动发酵单胞菌丙酮酸脱羧酶（pdc）和乳酸乳球菌NADH氧化酶（nox）的过表达，可实现葡萄糖向乙醛的高效转化。在静息细胞中，在厌氧条件下通过pdc和nox的联合过表达，几乎50%被消耗的葡萄糖可被重定向生成乙醛。
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