van Drunen Littel-van den Hurk S, Gerdts V, Loehr B I, Pontarollo R, Rankin R, Uwiera R, Babiuk L A
Veterinary Infectious Disease Organization, 120 Veterinary Road, Saskatoon, Saskatchewan S7N 5E3, Canada.
Adv Drug Deliv Rev. 2000 Sep 15;43(1):13-28. doi: 10.1016/s0169-409x(00)00074-0.
DNA-based vaccination constitutes one of the most recent approaches to vaccine development. This technology is in principle one of the most simple and yet versatile methods of inducing both humoral and cellular immune responses, as well as protection against a variety of infectious agents. However, although immune responses have been induced in a number of larger species, most information on the efficacy of DNA immunization has been generated in mice. In this review the information available to date about the use of DNA vaccines in farmed animals, including cattle, pigs and poultry, is presented. The areas that need specific attention in the future to bring this technology to the market are discussed, including the issues concerning delivery, safety, compatibility of plasmids in multivalent vaccines and the potential of using immune stimulants as part of a DNA vaccine.
基于DNA的疫苗接种是疫苗开发的最新方法之一。从原理上讲，这项技术是诱导体液免疫和细胞免疫反应以及抵御多种传染原的最简单且用途最广泛的方法之一。然而，尽管已在许多较大物种中诱导出免疫反应，但有关DNA免疫效力的大多数信息都是在小鼠身上获得的。在这篇综述中，介绍了迄今为止有关在养殖动物（包括牛、猪和家禽）中使用DNA疫苗的可用信息。讨论了未来要将这项技术推向市场需要特别关注的领域，包括与递送、安全性、多价疫苗中质粒的兼容性以及使用免疫刺激剂作为DNA疫苗一部分的潜力有关的问题。
