Dieterich P, Odenthal-Schnittler M, Mrowietz C, Krämer M, Sasse L, Oberleithner H, Schnittler H J
Rechenzentrum der Universität Würzburg, Am Hubland, 97074 Würzburg, Germany.
Biophys J. 2000 Sep;79(3):1285-97. doi: 10.1016/S0006-3495(00)76382-X.
To evaluate shear stress-induced effects on cultured cells we have extended the mechanical setup of a multichannel in vitro rheological system and developed software allowing entire processing control and image data analysis. The values of cell motility, degree of orientation (alignment), and cell elongation were correlated as a function of time (morphodynamics). Collective and individual endothelial cells within confluent cultures displayed a shear stress-dependent characteristic phase behavior of the following time course: resting conditions (phase I), change of motility (phase II), onset of alignment (phase III), and finally cell elongation (phase IV). Especially cell motility was characterized by a randomized zigzag movement around mean trajectories (fluctuations) together with mean cell locomotion. Onset of shear stress caused a down-regulation of fluctuations of 30% within <10 min and simultaneously increased locomotion velocities preferring the flow direction (phase II). After a lag period of 10 to 20 min cells orientated in the direction of flow (phase III) without significant cell elongation, which finally occurs within hours (phase IV). These data provide first evidence that cells within confluent endothelial monolayers respond to shear stress with a characteristic phase behavior.
为了评估剪切应力对培养细胞的影响，我们扩展了多通道体外流变系统的机械装置，并开发了允许进行完整过程控制和图像数据分析的软件。细胞运动性、定向程度（排列）和细胞伸长的值作为时间的函数（形态动力学）进行关联。汇合培养物中的集体和单个内皮细胞表现出以下时间进程的剪切应力依赖性特征相行为：静止状态（阶段I）、运动性变化（阶段II）、排列开始（阶段III），最后是细胞伸长（阶段IV）。特别是细胞运动性的特征是围绕平均轨迹的随机曲折运动（波动）以及平均细胞移动。剪切应力的施加在<10分钟内导致波动下调30%，同时增加了倾向于流动方向的移动速度（阶段II）。在10到20分钟的滞后时间后，细胞沿流动方向定向（阶段III），没有明显的细胞伸长，最终在数小时内发生（阶段IV）。这些数据提供了第一个证据，表明汇合内皮单层中的细胞以特征相行为对剪切应力作出反应。
