Van Noorden C J
Department of Cell Biology and Histology, Academic Medical Center, University of Amsterdam, The Netherlands.
Folia Histochem Cytobiol. 2000;38(3):99-102.
Oxygen insensitivity of cancer cells and oxygen sensitivity of non-cancer cells in the histochemical assay of glucose-6-phosphate dehydrogenase (G6PD) activity enables detection of cancer cells in unfixed cell smears or cryostat sections of biopsies. The assay is based on reduction of the tetrazolium salt neotetrazolium. It is a cheap assay that is easy to perform. It takes only 30 min at the most. The test discriminates between adenomas and carcinomas of colon and rectum with a certainty higher than 80% and is the best prognosticator of survival of colorectal cancer patients. Pancreatic cancer can be discriminated from pancreatitis with 100% certainty. Therefore, the assay is an excellent tool for the pathologist to provide additional information in difficult cases of diagnosis of cancer and for prognosis.
在葡萄糖-6-磷酸脱氢酶（G6PD）活性的组织化学测定中，癌细胞对氧气不敏感，而非癌细胞对氧气敏感，这使得在未固定的细胞涂片或活检的冷冻切片中能够检测到癌细胞。该测定基于四氮唑盐新四氮唑的还原反应。这是一种成本低廉且易于操作的测定方法。最多只需30分钟。该检测对结肠和直肠癌的腺瘤和癌的鉴别准确率高于80%，是结直肠癌患者生存的最佳预后指标。胰腺癌与胰腺炎的鉴别准确率可达100%。因此，该测定是病理学家在癌症诊断困难病例中提供额外信息和进行预后判断的极佳工具。
