一种用于从胃癌和结肠癌中识别恶性细胞的改良四氮唑反应。
A modified tetrazolium reaction for identifying malignant cells from gastric and colonic cancer.
作者信息
Ibrahim K S, Husain O A, Bitensky L, Chayen J
出版信息
J Clin Pathol. 1983 Feb;36(2):133-6. doi: 10.1136/jcp.36.2.133.
Abstract
When non-malignant cells were reacted for glucose-6-phosphate dehydrogenase activity, with neotetrazolium chloride as the indicator of the activity, oxygen competed with the neotetrazolium and nullified the reaction. In contrast, about 30% of the activity was retained in malignant cells, in sections and in smears, from cancer of the stomach or colon. This could provide the basis of a qualitative (black-or-white) functional test for distinguishing malignant cells in these conditions.
摘要
当使用新四氮唑氯化物作为活性指标，对非恶性细胞进行葡萄糖-6-磷酸脱氢酶活性反应时，氧气与新四氮唑竞争并使反应无效。相比之下，在来自胃癌或结肠癌的切片和涂片的恶性细胞中，约30%的活性得以保留。这可为在这些情况下区分恶性细胞的定性（黑白分明）功能测试提供基础。
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