Walter L, Günther E
Abteilung Immungenetik, Georg-August-Universität Göttingen, Germany.
Immunogenetics. 2000 Aug;51(10):829-37. doi: 10.1007/s002510000219.
We physically mapped the centromeric part of the BN rat MHC (RT1n haplotype) in a contig of overlapping P1-derived artificial chromosome (PAC) clones encompassing about 300 kb. The following genes were identified and ordered as: (Syngap, Hset, Daxx, Bing1)-Tapbp-Rgl2-Ke2-Bing4-B3galt4- Rps18-Sacm2l-RT1-A1-RT1-A2-RT1-A3-Ring1-Ring2-++ +Ke4-Rxrb-Col11a2-RT1-Hb-Ring3-RT1-DMb. Thus, in contrast to other RT1 haplotypes, RT1n contains three class I genes, RT1-A1, RT1-A2, and RT1-A3, mapping between the Sacm2l and Ring1 genes. Comparisons of the sequences flanking the Sacm2L and Ring1 genes in rat, human, and mouse suggest that the class I gene-containing region was inserted between these genes in rat and mouse at a similar position. Thus, this insertion is likely to have occurred in a common ancestor of these rodents, although the presence of a site particularly permissive for insertions cannot be excluded.
我们在一个由重叠的P1衍生人工染色体（PAC）克隆组成的重叠群中对BN大鼠主要组织相容性复合体（MHC，RT1n单倍型）的着丝粒部分进行了物理图谱绘制，该重叠群覆盖约300 kb。鉴定并排列了以下基因：（Syngap、Hset、Daxx、Bing1）-Tapbp-Rgl2-Ke2-Bing4-B3galt4-Rps18-Sacm2l-RT1-A1-RT1-A2-RT1-A3-Ring1-Ring2-++ +Ke4-Rxrb-Col11a2-RT1-Hb-Ring3-RT1-DMb。因此，与其他RT1单倍型不同，RT1n包含三个I类基因，即RT1-A1、RT1-A2和RT1-A3，定位于Sacm2l和Ring1基因之间。对大鼠、人类和小鼠中Sacm2L和Ring1基因侧翼序列的比较表明，含I类基因的区域在大鼠和小鼠中插入到这些基因之间的相似位置。因此，这种插入很可能发生在这些啮齿动物的共同祖先中，尽管不能排除存在一个对插入特别允许的位点。
