Caropreso S, Bondioli L, Capannolo D, Cerroni L, MacChiarelli R, Condò S G
School of Dentistry, Department of Experimental Medicine and Biological Science, University of Rome 'Tor Vergata', via di Tor Vergata 135, 00133 Rome, Italy. Anthropology, P.
J Microsc. 2000 Sep;199(Pt 3):244-7. doi: 10.1046/j.1365-2818.2000.00731.x.
We describe a simple method by which thin sections ( approximately 100 microm) from modern and archaeological teeth and bones can be obtained. A detailed embedding-cutting-mounting procedure is proposed, suggesting the use of a dental adhesive system, composite resins and conventional embedding resins, with the aims of improving the quality of the sections and substantially reducing the steps and time needed to prepare specimens for histological analysis. The introduction of this dental materials-based system allows an accurate positioning of the sample embedded inside the resin, prevents cracks and distortions of the section during the cutting phase and generally improves mounting sections on slides.
我们描述了一种简单的方法,通过该方法可以获取来自现代和考古牙齿及骨骼的薄片(约100微米)。提出了一种详细的包埋-切割-装片程序,建议使用牙科粘结系统、复合树脂和传统包埋树脂,目的是提高切片质量并大幅减少为组织学分析制备标本所需的步骤和时间。这种基于牙科材料的系统的引入能够精确地将包埋在树脂中的样品定位,防止在切割阶段切片出现裂缝和变形,并总体上改善切片在载玻片上的装片效果。