Kito M, Ishinaga M, Nishihara M, Kato M, Sawada S
Eur J Biochem. 1975 May;54(1):55-63. doi: 10.1111/j.1432-1033.1975.tb04113.x.
The fractionation, turnover and biosynthesis of the phosphatidylglycerol molecular species of Escherichia coli were studied. Monoacetyldiglycerides derived from phosphatidylglycerol were separated into five subfractions; cis-vaccenyl-palmitoleyl, cis-vaccenyl-cis-vaccenyl, palmityl-palmitoleyl, palmityl-cis-vaccenyl and the disaturated molecular species on a silica gel plate impregnated with silver nitrate. Individual molecular species had different turnover rates. The palmityl-cis-vaccenyl species was metabolized faster than the others. Disaturated species were rather stable. Various phosphatidylglycerol molecular species were synthesized in the presence of sn-glycerol 3-phosphate, palmityl-CoA, palmitoleyl-CoA, cis-vaccenyl-CoA and CTP by the E. coli membrane particulate fraction. When only the proportion of palmityl-CoA among the three acyl-CoAs was increased, the molecular species containing the palmityl residue were increased. Similar results were obtained with the other acyl-CoAs. However, a temperature-sensitive incorporation of unsaturated and saturated fatty acids into phosphatidylglycerol molecular species was observed with no change in the proportions of the three acyl-CoAs, completely reflecting the in vivo unsaturated/saturated ratio.
对大肠杆菌磷脂酰甘油分子种类的分级分离、周转和生物合成进行了研究。源自磷脂酰甘油的单乙酰二甘油酯被分离成五个亚级分；顺式-十八碳烯酰-棕榈油烯酰、顺式-十八碳烯酰-顺式-十八碳烯酰、棕榈酰-棕榈油烯酰、棕榈酰-顺式-十八碳烯酰以及在浸渍有硝酸银的硅胶板上的二饱和分子种类。各个分子种类具有不同的周转率。棕榈酰-顺式-十八碳烯酰种类比其他种类代谢得更快。二饱和种类相当稳定。大肠杆菌膜颗粒部分在sn-甘油3-磷酸、棕榈酰辅酶A、棕榈油烯酰辅酶A、顺式-十八碳烯酰辅酶A和CTP存在的情况下合成了各种磷脂酰甘油分子种类。当三种酰基辅酶A中仅棕榈酰辅酶A的比例增加时，含有棕榈酰残基的分子种类增加。用其他酰基辅酶A也得到了类似的结果。然而，在三种酰基辅酶A的比例没有变化的情况下，观察到不饱和脂肪酸和饱和脂肪酸向磷脂酰甘油分子种类的温度敏感掺入，这完全反映了体内不饱和/饱和比例。
