Nishihara M, Ishinaga M, Kato M, Kito M
Biochim Biophys Acta. 1976 Apr 22;431(1):54-61.
Phospholipids in the membranes of Escherichia coli grown at 37 degrees C are composed of different proportions of molecular species than are those at 17 degrees C. The 1,2-disaturated and 1-saturated-2-unsaturated molecular species increased at 37 degrees C, but the 1,2-diunsaturated species increased at 17 degrees C. When the growth temperature was lowered from 37 to 17 degrees C during the middle exponential growth phase, phosphatidylethanolamine and phosphatidylglycerol composed of proportions of molecular species similar to those found at 17 degrees C were immediately synthesized. By using various membranes composed of different compositions of the phospholipid molecular species, the temperature-sensitive formation of the phospholipid molecular species was found to be independent of the composition of the membrane phospholipids and to be dependent on changes in the specificities of membrane-bound sn-glycerol 3-phosphate acyltransferase against the acyl-CoAs, due to temperature changes.
在37摄氏度下生长的大肠杆菌膜中的磷脂，其分子种类的比例与在17摄氏度下生长的大肠杆菌不同。1,2-二饱和和1-饱和-2-不饱和分子种类在37摄氏度时增加，但1,2-二不饱和种类在17摄氏度时增加。当中指数生长期将生长温度从37摄氏度降至17摄氏度时，立即合成了由与在17摄氏度时发现的分子种类比例相似的磷脂酰乙醇胺和磷脂酰甘油。通过使用由不同磷脂分子种类组成的各种膜，发现磷脂分子种类的温度敏感性形成与膜磷脂的组成无关，并且取决于由于温度变化导致的膜结合的sn-甘油-3-磷酸酰基转移酶对酰基辅酶A的特异性变化。
