Doering L C, Snyder E Y
Department of Pathology and Molecular Medicine, Health Science Center, McMaster University, Hamilton, Ontario, Canada.
J Neurosci Res. 2000 Sep 15;61(6):597-604. doi: 10.1002/1097-4547(20000915)61:6<597::AID-JNR3>3.0.CO;2-L.
The potential of a neural stem cell line to acquire cholinergic characteristics was studied in transplants injected into the septum/diagonal band nuclei of young adult rats and mice. The stem cells integrated within the nuclei and survived for up to 9 months. Three methods were used to identify the grafted cells and to show differentiation into astrocytes and neurons. Enhanced survival of the stem cells occurred in the host brain with a previous lesion of the fimbria-fornix pathway. Differentiated cells acquired neuronal-like features including the expression of neurofilament subunits. In lesioned hosts, subpopulations of the grafted cells acquired a cholinergic neuronal phenotype and expressed choline acetyltransferase and the p75 neurotrophin receptor. Cells that developed into astrocytes were often associated with blood vessels and expressed glial fibrillary acidic protein. The results further exemplify the potential of stem cell lines and the property of site-specific differentiation when this line is transplanted to the cholinergic system of the adult brain.
在注射到年轻成年大鼠和小鼠的隔区/斜角带核的移植实验中，研究了一种神经干细胞系获得胆碱能特性的潜力。这些干细胞整合到核内，并存活长达9个月。使用了三种方法来鉴定移植的细胞，并显示其向星形胶质细胞和神经元的分化。在先前有穹窿-海马伞通路损伤的宿主脑中，干细胞的存活率有所提高。分化的细胞获得了神经元样特征，包括神经丝亚基的表达。在受损宿主中，移植细胞的亚群获得了胆碱能神经元表型，并表达胆碱乙酰转移酶和p75神经营养因子受体。发育为星形胶质细胞的细胞通常与血管相关，并表达胶质纤维酸性蛋白。这些结果进一步例证了干细胞系的潜力以及该细胞系移植到成年脑胆碱能系统时的位点特异性分化特性。
