Stein-Douglas K, Schwarting G A, Naiki M, Marcus D M
J Exp Med. 1976 Apr 1;143(4):822-32. doi: 10.1084/jem.143.4.822.
Antibodies to GM1 ganglioside were used to study murine lymphocyte populations. In A, AKR, and BALB/c mice, anti-GM1 reacts with thymocytes and peripheral T cells. This reactivity of anti-GM1, studied by immunofluorescence, is independent of Thy-1 type and appears to be related to the reactivity of cross-reacting antibodies to asialo GM1 and GD1b, rather than GM1 itself. In addition, a subpopulation of lymphocytes reacting with anti-GM1 and anti-immunoglobulin has been found in approximately 26% of the peripheral lymphocytes of C3H mice, nude mice, and nude heterozygotes. This subpopulation is found in small numbers in A, AKR, and BALB/c mice. These studies demonstrate that antibodies to a chemically defined antigen can be used to identify T cells in many strains of mice and may delineate previously unrecognized lymphocyte subpopulations.
利用针对GM1神经节苷脂的抗体来研究小鼠淋巴细胞群体。在A、AKR和BALB/c小鼠中，抗GM1与胸腺细胞和外周T细胞发生反应。通过免疫荧光研究发现，抗GM1的这种反应性与Thy-1类型无关，似乎与抗唾液酸GM1和GD1b的交叉反应抗体的反应性有关，而非与GM1本身有关。此外，在C3H小鼠、裸鼠和裸杂合子约26%的外周淋巴细胞中发现了一个与抗GM1和抗免疫球蛋白发生反应的淋巴细胞亚群。在A、AKR和BALB/c小鼠中，该亚群数量较少。这些研究表明，针对化学定义抗原的抗体可用于识别多种小鼠品系中的T细胞，并可能描绘出先前未被识别的淋巴细胞亚群。
