构巢曲霉中NIMA激酶介导的有丝分裂组蛋白H3磷酸化作用

Mitotic histone H3 phosphorylation by the NIMA kinase in Aspergillus nidulans.

作者信息

De Souza C P, Osmani A H, Wu L P, Spotts J L, Osmani S A

机构信息

Henry Hood Research Program, Weis Center for Research, Geisinger Clinic, Danville, Pennsylvania 17822, USA.

出版信息

Cell. 2000 Aug 4;102(3):293-302. doi: 10.1016/s0092-8674(00)00035-0.

DOI:10.1016/s0092-8674(00)00035-0

PMID:10975520

Abstract

Phosphorylation of histone H3 serine 10 correlates with chromosome condensation and is required for normal chromosome segregation in Tetrahymena. This phosphorylation is dependent upon activation of the NIMA kinase in Aspergillus nidulans. NIMA expression also induces Ser-10 phosphorylation inappropriately in S phase-arrested cells and in the absence of NIMX(cdc2) activity. At mitosis, NIMA becomes enriched on chromatin and subsequently localizes to the mitotic spindle and spindle pole bodies. The chromatin-like localization of NIMA early in mitosis is tightly correlated with histone H3 phosphorylation. Finally, NIMA can phosphorylate histone H3 Ser-10 in vitro, suggesting that NIMA is a mitotic histone H3 kinase, perhaps helping to explain how NIMA promotes chromatin condensation in A. nidulans and when expressed in other eukaryotes.

摘要

组蛋白H3丝氨酸10的磷酸化与染色体凝聚相关，并且是嗜热四膜虫正常染色体分离所必需的。这种磷酸化依赖于构巢曲霉中NIMA激酶的激活。NIMA的表达还会在S期停滞的细胞中以及在缺乏NIMX（cdc2）活性的情况下不恰当地诱导Ser-10磷酸化。在有丝分裂时，NIMA在染色质上富集，随后定位于有丝分裂纺锤体和纺锤极体。有丝分裂早期NIMA类似染色质的定位与组蛋白H3磷酸化紧密相关。最后，NIMA在体外可以磷酸化组蛋白H3丝氨酸10，这表明NIMA是一种有丝分裂组蛋白H3激酶，这或许有助于解释NIMA如何在构巢曲霉中促进染色质凝聚以及在其他真核生物中表达时的作用。