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1
In vitro selection and extended culture of antigen-specific T lymphocytes. I. Description of selection culture procedure and initial characterization of selected cells.抗原特异性T淋巴细胞的体外选择与扩增培养。I. 选择培养程序的描述及所选细胞的初步表征。
J Exp Med. 1975 Jul 1;142(1):90-105. doi: 10.1084/jem.142.1.90.
2
In vitro selection and extended culture of antigen-specific T lymphocytes. II. Mechanisms of selection.抗原特异性T淋巴细胞的体外选择与长期培养。II. 选择机制。
J Immunol. 1975 Dec;115(6):1723-30.
3
The specificity of cellular immune responses in guinea pigs. I. T cells specific for 2,4-dinitrophenyl-o-tyrosyl residues.豚鼠细胞免疫反应的特异性。I. 对2,4-二硝基苯基-o-酪氨酰残基特异的T细胞
J Exp Med. 1975 Jan 1;141(1):42-55. doi: 10.1084/jem.141.1.42.
4
Independent populations of primed F1 guinea pig T lymphocytes respond to antigen-pulsed parental peritoneal exudate cells.致敏的F1豚鼠T淋巴细胞的独立群体对经抗原刺激的亲代腹膜渗出细胞产生反应。
J Exp Med. 1977 Mar 1;145(3):618-30. doi: 10.1084/jem.145.3.618.
5
Macrophage control of time-dependent changes in antigen sensitivity of immune T lymphocyte populations.巨噬细胞对免疫性T淋巴细胞群体抗原敏感性随时间变化的控制。
J Immunol. 1974 Jan;112(1):359-69.
6
Lymphocyte proliferation in vitro induced by hapten autologous protein conjugates. I. A study on the class of lymphocytes responding in vitro and on the nature and specificity of their receptors.半抗原自体蛋白缀合物诱导的体外淋巴细胞增殖。I. 体外应答淋巴细胞类别及其受体性质和特异性的研究
J Exp Med. 1974 Mar 1;139(3):732-53. doi: 10.1084/jem.139.3.732.
7
Antigen-macrophage interaction. I. Hapten-specific inhibition of antigen interaction with macrophages from immune animals.抗原-巨噬细胞相互作用。I. 半抗原特异性抑制免疫动物来源的巨噬细胞与抗原的相互作用。
J Immunol. 1973 Sep;111(3):811-9.
8
Biochemical characterization of Ia alloantigens in guinea pigs. I. Synthesis of Ia antigens by subsets enriched for B cells, T cells, or macrophages.豚鼠Ia同种异体抗原的生化特性。I. 富含B细胞、T细胞或巨噬细胞的亚群对Ia抗原的合成。
J Immunol. 1981 Nov;127(5):1879-85.
9
Alloantiserum-induced inhibition of immune response gene product function. I. Cellular distribution of target antigens.同种抗血清诱导的免疫应答基因产物功能抑制。I. 靶抗原的细胞分布
J Exp Med. 1974 Mar 1;139(3):661-78. doi: 10.1084/jem.139.3.661.
10
Genetic restriction in T-lymphocyte activation by antigen-pulse peritoneal exudate cells.
Cold Spring Harb Symp Quant Biol. 1977;41 Pt 2:571-8. doi: 10.1101/sqb.1977.041.01.066.

引用本文的文献

1
Revisiting the first long-term culture of antigen-specific cytotoxic T cells.重温抗原特异性细胞毒性T细胞的首次长期培养。
Front Immunol. 2014 May 5;5:194. doi: 10.3389/fimmu.2014.00194. eCollection 2014.
2
Toward a molecular understanding of adaptive immunity: a chronology, part I.朝着对适应性免疫的分子理解迈进：一个年表，第一部分。
Front Immunol. 2012 Dec 6;3:369. doi: 10.3389/fimmu.2012.00369. eCollection 2012.
3
Antigen-pulsed macrophage monolayers as specific immunoabsorbents: selective absorption of murine T cells committed to soluble protein antigen.作为特异性免疫吸附剂的抗原脉冲巨噬细胞单层：对可溶性蛋白抗原有特异性的小鼠T细胞的选择性吸收
Immunology. 1981 Jul;43(3):555-62.
4
Independent populations of primed F1 guinea pig T lymphocytes respond to antigen-pulsed parental peritoneal exudate cells.致敏的F1豚鼠T淋巴细胞的独立群体对经抗原刺激的亲代腹膜渗出细胞产生反应。
J Exp Med. 1977 Mar 1;145(3):618-30. doi: 10.1084/jem.145.3.618.
5
The histocompatibility restrictions on macrophage T-helper cell interaction determine the histocompatibility restrictions on T-helper cell B-cell interaction.巨噬细胞与辅助性T细胞相互作用中的组织相容性限制决定了辅助性T细胞与B细胞相互作用中的组织相容性限制。
J Exp Med. 1978 Nov 1;148(5):1171-85. doi: 10.1084/jem.148.5.1171.
6
The role of H-2 linked genes in helper T-cell function. II. Isolation on antigen-pulsed macrophages of two separate populations of F1 helper T cells each specific for antigen and one set of parental H-2 products.H-2连锁基因在辅助性T细胞功能中的作用。II. 在抗原脉冲巨噬细胞上分离出两个不同的F1辅助性T细胞群体，每个群体对抗原和一组亲本H-2产物具有特异性。
J Exp Med. 1978 Feb 1;147(2):554-70. doi: 10.1084/jem.147.2.554.

本文引用的文献

1
Studies on artifical antigens. I. Antigenicity of DNP-polylysine and DNP copolymer of lysine and glutamic acid in guinea pigs.人工抗原的研究。I. 二硝基苯-聚赖氨酸及赖氨酸与谷氨酸的二硝基苯共聚物在豚鼠体内的抗原性
J Exp Med. 1963 Jan 1;117(1):55-69. doi: 10.1084/jem.117.1.55.
2
Cell separation on antigen-coated columns. Elimination of high rate antibody-forming cells and immunological memory cells.抗原包被柱上的细胞分离。去除高速率抗体形成细胞和免疫记忆细胞。
J Exp Med. 1969 Jan 1;129(1):23-36. doi: 10.1084/jem.129.1.23.
3
Complex specificity of immune lymphocytes in allogeneic cell cultures.同种异体细胞培养中免疫淋巴细胞的复杂特异性
Folia Biol (Praha). 1968;14(2):115-31.
4
Receptors on immunocompetent cells. I. Receptor specificity of cells participating in a cellular immune response.免疫活性细胞上的受体。I. 参与细胞免疫应答的细胞的受体特异性。
Cell Immunol. 1970 Oct;1(4):404-18. doi: 10.1016/0008-8749(70)90017-1.
5
The peritoneal exudate lymphocyte. I. Differences in antigen responsiveness between peritoneal exudate and lymph node lymphocytes from immunized guinea pigs.腹膜渗出淋巴细胞。I. 免疫豚鼠腹膜渗出淋巴细胞与淋巴结淋巴细胞抗原反应性的差异。
J Exp Med. 1971 Nov 1;134(5):1170-86. doi: 10.1084/jem.134.5.1170.
6
Cells mediating specific in vitro cytotoxicity. I. Detection of receptor-bearing lymphocytes.介导特异性体外细胞毒性的细胞。I. 携带受体淋巴细胞的检测。
J Exp Med. 1971 Dec 1;134(6):1385-402. doi: 10.1084/jem.134.6.1385.
7
Synergistic interaction of macrophages and lymphocytes in antigen-induced transformation of lymphocytes.巨噬细胞与淋巴细胞在抗原诱导的淋巴细胞转化中的协同相互作用。
J Exp Med. 1970 Jul 1;132(1):44-65. doi: 10.1084/jem.132.1.44.
8
Receptors on immunocompetent cells. II. Specificity and nature of receptors on dinitrophenylated guinea pig albumin- 125 I-binding lymphocytes of normal guinea pigs.免疫活性细胞上的受体。II. 正常豚鼠二硝基苯基化豚鼠白蛋白-125I结合淋巴细胞上受体的特异性和性质。
J Exp Med. 1971 Aug 1;134(2):495-516. doi: 10.1084/jem.134.2.495.
9
A simple and versatile harvesting device for processing radioactive label incorporated into and-or released from cells in microculture.一种简单且通用的收获装置，用于处理微量培养中掺入细胞和/或从细胞中释放的放射性标记物。
J Immunol Methods. 1974 Jan;3(1):11-6. doi: 10.1016/0022-1759(74)90027-1.
10
Hapten-specific augmentation of the anti-idiotype antibody response to hapten-myeloma protein conjugates in mice.半抗原特异性增强小鼠对半抗原-骨髓瘤蛋白缀合物的抗独特型抗体反应。
Eur J Immunol. 1973 Jun;3(6):340-7. doi: 10.1002/eji.1830030605.

抗原特异性T淋巴细胞的体外选择与扩增培养。I. 选择培养程序的描述及所选细胞的初步表征。

In vitro selection and extended culture of antigen-specific T lymphocytes. I. Description of selection culture procedure and initial characterization of selected cells.

作者信息

Ben-Sasson S Z, Paul W E, Shevach E M, Green I

出版信息

J Exp Med. 1975 Jul 1;142(1):90-105. doi: 10.1084/jem.142.1.90.

DOI:10.1084/jem.142.1.90
PMID:1097577
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2189876/
Abstract

Specific selection of antigen-responsive guinea pig peritoneal exudate lymphocytes (PELs) was achieved by a selection culture procedure. This procedure involved the addition of PELs from immune donors to monolayers of antigen-pulsed adherent peritoneal exudate cells from nonprimed syngeneic donors. PELs which failed to adhere were discarded at 24 and 48 h; after 1 wk of culture, lymphocytes were obtained which were highly responsive to the antigen for which they were selected but which demonstrated little or no response to other antigens to which the original donor of the lymphocyte was immune. These selected cells were largely T lymphocytes and could be maintained in culture for 2-5 wk in an antigen-responsive state and, in 20-30% of cases, for 8-10 wk in an antigen-independent state.

摘要

通过一种选择培养程序实现了对抗原反应性豚鼠腹腔渗出淋巴细胞（PEL）的特异性选择。该程序包括将来自免疫供体的PEL添加到来自未致敏同基因供体的抗原脉冲贴壁腹腔渗出细胞单层中。未黏附的PEL在24小时和48小时时被丢弃；培养1周后，获得了对其被选择的抗原具有高度反应性但对淋巴细胞原始供体免疫的其他抗原几乎没有或没有反应的淋巴细胞。这些被选择的细胞主要是T淋巴细胞，可以在抗原反应状态下在培养中维持2 - 5周，并且在20 - 30%的情况下，可以在抗原非依赖状态下维持8 - 10周。