抗原特异性T淋巴细胞的体外选择与扩增培养。I. 选择培养程序的描述及所选细胞的初步表征。
In vitro selection and extended culture of antigen-specific T lymphocytes. I. Description of selection culture procedure and initial characterization of selected cells.
作者信息
Ben-Sasson S Z, Paul W E, Shevach E M, Green I
出版信息
J Exp Med. 1975 Jul 1;142(1):90-105. doi: 10.1084/jem.142.1.90.
Abstract
Specific selection of antigen-responsive guinea pig peritoneal exudate lymphocytes (PELs) was achieved by a selection culture procedure. This procedure involved the addition of PELs from immune donors to monolayers of antigen-pulsed adherent peritoneal exudate cells from nonprimed syngeneic donors. PELs which failed to adhere were discarded at 24 and 48 h; after 1 wk of culture, lymphocytes were obtained which were highly responsive to the antigen for which they were selected but which demonstrated little or no response to other antigens to which the original donor of the lymphocyte was immune. These selected cells were largely T lymphocytes and could be maintained in culture for 2-5 wk in an antigen-responsive state and, in 20-30% of cases, for 8-10 wk in an antigen-independent state.
摘要
通过一种选择培养程序实现了对抗原反应性豚鼠腹腔渗出淋巴细胞（PEL）的特异性选择。该程序包括将来自免疫供体的PEL添加到来自未致敏同基因供体的抗原脉冲贴壁腹腔渗出细胞单层中。未黏附的PEL在24小时和48小时时被丢弃；培养1周后，获得了对其被选择的抗原具有高度反应性但对淋巴细胞原始供体免疫的其他抗原几乎没有或没有反应的淋巴细胞。这些被选择的细胞主要是T淋巴细胞，可以在抗原反应状态下在培养中维持2 - 5周，并且在20 - 30%的情况下，可以在抗原非依赖状态下维持8 - 10周。
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