Li J, Thornhill M H
Oral Disease Research Centre, St Bartholomew's and The Royal London School of Medicine and Dentistry, London, UK.
Cytokine. 2000 Sep;12(9):1409-13. doi: 10.1006/cyto.2000.0713.
Growth regulated peptide (GRO-alpha) is chemotactic for neutrophils. It also stimulates keratinocyte proliferation and migration, and angiogenesis in cutaneous wound healing. We compared GRO-alpha production by normal human skin and oral keratinocytes, and the effects of cytokine stimulation. Resting keratinocytes produced little, if any, GRO-alpha. TNF-alpha induced a large increase in GRO-alpha mRNA and protein production in both cell types (P<0.001). However, the response of oral keratinocytes was significantly higher (P<0.01). Oral, but not skin, keratinocytes also produced significant amounts of GRO-alpha in response to IL-1 alpha (P<0.005) and IL-4 (P<0.01) stimulation. Indeed, there was an additive effect on GRO-alpha production when oral keratinocytes were stimulated with combinations of TNF-alpha and IL-1 alpha or TNF-alpha and IL-4. Neither cell type responded to interferon gamma. Keratinocyte GRO-alpha production may help selectively recruit neutrophils in mucocutaneous inflammatory diseases, and differences in production by skin and oral keratinocyte could explain the different presentation of these diseases at the two sites. The increased GRO-alpha responsiveness of oral keratinocytes may also help explain the excellent wound healing properties of oral mucosa.
生长调节肽（GRO-α）对中性粒细胞具有趋化作用。它还能刺激角质形成细胞增殖和迁移，并在皮肤伤口愈合过程中促进血管生成。我们比较了正常人皮肤角质形成细胞和口腔角质形成细胞产生GRO-α的情况，以及细胞因子刺激的影响。静息的角质形成细胞几乎不产生GRO-α。肿瘤坏死因子-α（TNF-α）可使两种细胞类型中的GRO-α mRNA和蛋白产量大幅增加（P<0.001）。然而，口腔角质形成细胞的反应明显更高（P<0.01）。口腔角质形成细胞而非皮肤角质形成细胞在白细胞介素-1α（IL-1α）（P<0.005）和白细胞介素-4（IL-4）（P<0.01）刺激下也会产生大量GRO-α。实际上，当用TNF-α与IL-1α或TNF-α与IL-4的组合刺激口腔角质形成细胞时，对GRO-α的产生有累加效应。两种细胞类型对干扰素γ均无反应。角质形成细胞产生GRO-α可能有助于在黏膜皮肤炎症性疾病中选择性募集中性粒细胞，皮肤和口腔角质形成细胞产生量的差异可以解释这些疾病在两个部位的不同表现。口腔角质形成细胞对GRO-α反应性的增加也可能有助于解释口腔黏膜出色的伤口愈合特性。
