Matsuura K, Otsuka F, Fujisawa H
Department of Dermatology, University of Tsukuba, Ibaraki, Japan.
Cytokine. 1998 Jul;10(7):500-5. doi: 10.1006/cyto.1997.0326.
Interferons (IFNs) have been reported to have pleiotrophic effects including the ability to induce the production of other cytokines in several cell types. Tumour necrosis factor alpha (TNF-alpha) is pro-inflammatory cytokine a known to be produced by a variety of cells including human keratinocytes. In the present study, we sought to determine the effects of IFNs on TNF-alpha production from human keratinocytes. IFN-gamma (50-100 ng/ml) induced TNF-alpha production dose dependently, but no induction of TNF-alpha was observed with IFN-alpha or IFN-beta. Since in the epidermis cytokines often work with in a cascade fashion and keratinocytes are a source of primary cytokine, IL-1 alpha, whether combined treatment with IFN-gamma and IL-1 alpha had a synergistic effect on TNF-alpha production was examined. Combined treatment with IFN-gamma (100 ng/ml) and IL-1 alpha (10 ng/ml) induced 2-3-fold higher level of TNF-alpha than IL-1 alpha alone. These results suggest that IFN-gamma is a positive regulator for the production of TNF-alpha from human keratinocytes and likely to increase skin inflammation.
据报道，干扰素（IFNs）具有多效性作用，包括能够在多种细胞类型中诱导其他细胞因子的产生。肿瘤坏死因子α（TNF-α）是一种促炎细胞因子，已知由包括人角质形成细胞在内的多种细胞产生。在本研究中，我们试图确定干扰素对人角质形成细胞产生TNF-α的影响。干扰素-γ（50-100 ng/ml）剂量依赖性地诱导TNF-α的产生，但未观察到干扰素-α或干扰素-β诱导TNF-α产生。由于在表皮中细胞因子通常以级联方式发挥作用，且角质形成细胞是主要细胞因子白细胞介素-1α的来源，因此研究了干扰素-γ与白细胞介素-1α联合治疗对TNF-α产生是否具有协同作用。干扰素-γ（100 ng/ml）与白细胞介素-1α（10 ng/ml）联合治疗诱导的TNF-α水平比单独使用白细胞介素-1α高2-3倍。这些结果表明，干扰素-γ是人角质形成细胞产生TNF-α的正调节因子，可能会加剧皮肤炎症。
