Traves S L, Culpitt S V, Russell R E K, Barnes P J, Donnelly L E
Department of Thoracic Medicine, National Heart and Lung Institute, Imperial College, School of Medicine, Dovehouse Street, London SW3 6LY, UK.
Thorax. 2002 Jul;57(7):590-5. doi: 10.1136/thorax.57.7.590.
Patients with chronic obstructive pulmonary disease (COPD) have increased numbers of neutrophils and macrophages in their lungs. Growth related oncogene-alpha (GROalpha) attracts neutrophils, whereas monocyte chemoattractant protein-1 (MCP-1) attracts monocytes that can differentiate into macrophages. The aim of this study was to determine the concentration of GROalpha and MCP-1 in bronchoalveolar lavage (BAL) fluid and sputum from non-smokers, healthy smokers and patients with COPD, and to see if there was a correlation between the concentrations of these chemokines, lung function, and numbers of inflammatory cells.
BAL fluid and sputum from non-smokers (n=32), healthy smokers (n=36), and patients with COPD (n=40) were analysed for the presence of GROalpha and MCP-1 using ELISA. Cells counts were performed on the samples and correlations between the concentrations of these chemokines, lung function, and inflammatory cells observed.
Median (SE) GROalpha and MCP-1 levels were significantly increased in sputum from patients with COPD compared with non-smokers and healthy smokers (GROalpha: 31 (11) v 2 (2) v 3 (0.8) ng/ml; MCP-1: 0.8 (0.4) v 0.2 (0.1) v 0.1 (0.04) ng/ml, p<0.05), but not in BAL fluid. There were significant negative correlations between both GROalpha and MCP-1 levels in sputum and forced expiratory volume in 1 second (FEV(1)) % predicted (GROalpha: r=-0.5, p<0.001; MCP-1: r=-0.5, p<0.001), together with significant positive correlations between GROalpha and MCP-1 and neutrophil numbers in sputum (GROalpha: r=0.6, p<0.001; MCP-1: r=0.4, p<0.01).
These results suggest that GROalpha and MCP-1 are involved in the migration of inflammatory cells, thus contributing to the inflammatory load associated with COPD.
慢性阻塞性肺疾病（COPD）患者肺部的中性粒细胞和巨噬细胞数量增加。生长相关癌基因α（GROα）吸引中性粒细胞，而单核细胞趋化蛋白-1（MCP-1）吸引可分化为巨噬细胞的单核细胞。本研究的目的是测定非吸烟者、健康吸烟者和COPD患者支气管肺泡灌洗（BAL）液和痰液中GROα和MCP-1的浓度，并观察这些趋化因子的浓度、肺功能和炎症细胞数量之间是否存在相关性。
使用酶联免疫吸附测定（ELISA）分析非吸烟者（n = 32）、健康吸烟者（n = 36）和COPD患者（n = 40）的BAL液和痰液中GROα和MCP-1的存在情况。对样本进行细胞计数，并观察这些趋化因子的浓度、肺功能和炎症细胞之间的相关性。
与非吸烟者和健康吸烟者相比，COPD患者痰液中的GROα和MCP-1水平中位数（SE）显著升高（GROα：31（11）对2（2）对3（0.8）ng/ml；MCP-1：0.8（0.4）对0.2（0.1）对0.1（0.04）ng/ml，p<0.05），但在BAL液中未升高。痰液中GROα和MCP-1水平与预测的1秒用力呼气量（FEV₁）%均呈显著负相关（GROα：r = -0.5，p<0.001；MCP-1：r = -0.5，p<0.001），同时GROα和MCP-1与痰液中的中性粒细胞数量呈显著正相关（GROα：r = 0.6，p<0.001；MCP-1：r = 0.4，p<0.01）。
这些结果表明，GROα和MCP-1参与炎症细胞的迁移，从而导致与COPD相关的炎症负荷增加。
