Kirkland J J, Truszkowski F A, Dilks C H, Engel G S
Agilent Technologies, Inc., Little Falls Chemical Analysis Group, Newport Site, DE 19804, USA.
J Chromatogr A. 2000 Aug 18;890(1):3-13. doi: 10.1016/s0021-9673(00)00392-7.
Very fast reversed-phase separations of biomacromolecules are performed using columns made with superficially porous silica microsphere column packings ("Poroshell"). These column packings consist of ultra-pure "biofriendly' silica microspheres composed of solid cores and thin outer shells with uniform pores. The excellent kinetic properties of these new column packings allow stable, high-resolution gradient chromatography of polypeptides, proteins, nucleic acids, DNA fragments, etc. in a fraction of the time required for conventional separations. Contrasted with <2-microm non-porous particles, Poroshell packings can be used optimally with existing equipment and greater sample loading capacities, while retaining kinetic (and separation speed) advantages over conventional totally porous particles.
使用由表面多孔硅胶微球柱填料（“Poroshell”）制成的色谱柱，可以对生物大分子进行非常快速的反相分离。这些柱填料由超纯的“生物友好型”硅胶微球组成，这些微球由实心核和具有均匀孔隙的薄外壳组成。这些新型柱填料优异的动力学性能，使得在传统分离所需时间的一小部分内，就能对多肽、蛋白质、核酸、DNA片段等进行稳定、高分辨率的梯度色谱分析。与<2微米的无孔颗粒相比，Poroshell填料可以与现有设备实现最佳配合，并具有更大的进样量，同时在动力学（和分离速度）方面优于传统的全多孔颗粒。
