Vito C C, Dowell C E
J Virol. 1976 Jun;18(3):942-9. doi: 10.1128/JVI.18.3.942-949.1976.
A capsid mutant of bacteriophage phi chi 174 demonstrates altered requirements for the conversion of viral single-stranded DNA to double-stranded replicative form DNA. In the presence of puromycin at 42 C, wild-type phi chi 174 is unable to complete this replicative event, whereas phi chi ahb is able to do so. Furthermore, in contrast to wild-type phi chi 174, formation of phi chi ahb parental replicative form DNA is sensitive to rifampin under certain experimental conditions. These data suggest that the mutant capsid proteins of phi chi ahb influence the biosynthesis of phi chi ahb complementary strand DNA.
噬菌体φX174的一种衣壳突变体显示出对病毒单链DNA转化为双链复制型DNA的需求发生了改变。在42℃下存在嘌呤霉素时,野生型φX174无法完成这一复制过程,而φX ahb则能够完成。此外,与野生型φX174不同,在某些实验条件下,φX ahb亲本复制型DNA的形成对利福平敏感。这些数据表明,φX ahb的突变衣壳蛋白影响了φX ahb互补链DNA的生物合成。