Othman Othman E, Abd El-Kader Heba A M, Alam Sally S, Abd El-Aziem Sekena H
Cell Biology Department, National Research Centre, Dokki, Egypt.
J Genet Eng Biotechnol. 2017 Jun;15(1):1-6. doi: 10.1016/j.jgeb.2017.04.006. Epub 2017 May 5.
This study was aimed to assess cytochrome b conservation in six breeds of camels reared in Egypt and to compare its sequence with those of other livestock species. The 208-bp fragments from camel mtDNA cyto b were amplified using PCR for 54 camels belonging to 6 camel breeds reared in Egypt. The alignment of camel cyto b sequences showed the presence of two polymorphic sites resulting in four haplotypes and their nucleotide sequences were submitted to GenBank under the accession numbers: KX909894-KX909897. The genetic distances between tested camel breeds were zero between Baladi, Fallahi and Maghrabi breeds whereas they were at low value between the other three breeds: Mowaled, Sodany and Somali. Neighbor-joining showed 4 branches; one of them include most of the tested animals and another one contains 2 Somali animals which is considered a specific haplotype for this breed. The other two branches are mixed between Sodani and Mowaled breeds. Neighbor-joining tree was constructed between cyto b sequences of our tested camels and their sequences from livestock species include Camelus dromedaries, Camelus bactrianus, Ovis aries, Capra hircus, Bubalus bubalis, Bos Taurus and Sus scrofa. The result confirmed that our camel breeds belong to Camelus dromedaries and are clearly separated from other species. It is concluded that cyto b sequence is highly conserved among all camel breeds reared in Egypt which belong to in addition to the advantage of cyto b in differentiation between different livestock sources which enables it to widely use for the adulteration detection in mixed meat.
本研究旨在评估在埃及饲养的六个骆驼品种中细胞色素b的保守性，并将其序列与其他家畜物种的序列进行比较。使用聚合酶链反应（PCR）对在埃及饲养的6个骆驼品种的54头骆驼的骆驼线粒体DNA细胞色素b的208个碱基对片段进行扩增。骆驼细胞色素b序列的比对显示存在两个多态性位点，产生了四种单倍型，其核苷酸序列已提交到GenBank，登录号为：KX909894 - KX909897。测试的骆驼品种之间，巴拉迪、法拉希和马格里布品种之间的遗传距离为零，而其他三个品种：穆瓦利德、索达尼和索马里之间的遗传距离较低。邻接法显示有4个分支；其中一个分支包括大多数测试动物，另一个分支包含2头索马里动物，这被认为是该品种的一个特定单倍型。另外两个分支是索达尼和穆瓦利德品种的混合。在我们测试的骆驼的细胞色素b序列与来自家畜物种（包括单峰驼、双峰驼、绵羊、山羊、水牛、黄牛和野猪）的序列之间构建了邻接树。结果证实我们的骆驼品种属于单峰驼，并且与其他物种明显分开。得出的结论是，细胞色素b序列在埃及饲养的所有骆驼品种中高度保守，除了细胞色素b在区分不同家畜来源方面的优势外，这使得它能够广泛用于混合肉中掺假检测。
