Tsuchida S, Ikemoto S
Department of Legal Medicine and Human Genetics, Jichi Medical School, Tochigi-ken, Japan.
J Vet Med Sci. 1992 Jun;54(3):417-24. doi: 10.1292/jvms.54.417.
Mitochondrial DNA (mtDNA) polymorphism was studied in 20 mongrel dogs using 14 restriction enzymes. The polymorphism was observed in the cleavage patterns of Apa I, EcoR I, EcoR V, Hinc II and Sty I. Three morphs using EcoR I and Apa I and two morphs using EcoR V, Hinc II and Sty I were found. However, no polymorphism was detected in the cleavage patterns of BamH I, Bgl II, Hae II, Hind III, Pst I, Sca I, Stu I and Xba I. The examined dogs were classified in seven types using five restriction endonucleases which recognized six nucleotide sequences. The value of nucleotide diversity was estimated to be 0.0055. A phylogenetic tree constructed by genetic distances among seven restriction types showed at least two clusters of mtDNA.
使用14种限制性内切酶对20只杂种犬的线粒体DNA（mtDNA）多态性进行了研究。在Apa I、EcoR I、EcoR V、Hinc II和Sty I的切割模式中观察到了多态性。发现使用EcoR I和Apa I时有三种形态，使用EcoR V、Hinc II和Sty I时有两种形态。然而，在BamH I、Bgl II、Hae II、Hind III、Pst I、Sca I、Stu I和Xba I的切割模式中未检测到多态性。使用识别六种核苷酸序列的五种限制性内切酶将所检查的犬分为七种类型。核苷酸多样性值估计为0.0055。由七种限制性类型之间的遗传距离构建的系统发育树显示至少有两个mtDNA簇。
