Sá-Leão R, Tomasz A, Sanches I S, Brito-Avô A, Vilhelmsson S E, Kristinsson K G, de Lencastre H
Molecular Genetics Unit, Instituto de Tecnologia Química e Biológica da Universidade Nova de Lisboa, Oeiras, Portugal.
J Infect Dis. 2000 Oct;182(4):1153-60. doi: 10.1086/315813. Epub 2000 Sep 1.
Over half (259/503) of drug-resistant (DR) pneumococci colonizing healthy children attending day care centers in Lisbon were identified by molecular typing methods as representatives of several internationally spread clones. These included the 2 penicillin-resistant pandemic Spanish/USA and French/Spanish clones (21% of all DR pneumococci) and 5 new lineages with unusual antibiotypes (accounting for an additional 30% of all DR pneumococci). The most characteristic feature of the latter group was the high frequency of resistance to macrolides and tetracycline and very low or no resistance to penicillin. These observations provide support for the notion that the nasopharyngeal flora of children in day care centers may be a global reservoir of worldwide prevalent strains of DR pneumococci.
通过分子分型方法鉴定出，在里斯本日托中心接受护理的健康儿童中，超过半数（259/503）的耐药肺炎球菌是几种国际传播克隆的代表菌株。其中包括两种耐青霉素的大流行西班牙/美国克隆株和法国/西班牙克隆株（占所有耐药肺炎球菌的21%），以及5个具有不寻常抗菌谱型的新谱系（占所有耐药肺炎球菌的另外30%）。后一组最显著的特征是对大环内酯类和四环素耐药的频率很高，而对青霉素耐药性很低或无耐药性。这些观察结果支持了这样一种观点，即日托中心儿童的鼻咽菌群可能是全球流行的耐药肺炎球菌菌株的全球储存库。
